The incidence of periodontitis which is varied in different countries around the world shows an increasing tendency in lipopolysaccharide (LPS) of Actinobacillus actinomycetemcomitans which is part of the cell wall of one of the virulence factors. The purpose of this study is to obtain and to characterize LPS of Surabaya isolates A. actinomycetemcomitans as the main cause of aggressive periodontitis and and to analyze the effect of LPS on the induction of IgA and IgG titers in serum and saliva. The method used in this study uses phenol to obtain and to characterize and purification LPS A. actinomycetemcomitans. Furthermore, it conducted in experimental animals (Wistar rats). This is done by calculating the levels of IgA, sIgA, and IgG with ELISA technique. This study results LPS of A. actinomycetemcomitans which is in accordance with LPS of E. coli O127 which is the standard of LPS. In experimental animals, the results show that the level of IgA in serum has increased although there is no significant difference with the control group from the statistical test, whereas the level of IgA in saliva shows significant differences if they are compared from the control group and the treatment group. The level of IgG in the treatment group also seems increasing although the statistical analysis did not show any significant difference from the controlled group. LPS of A. actinomycetemcomitans can increase the antibody of Wistar rats, especially the level of IgA in saliva (sIgA). This suggests that sIgA roles in local immune defense.
|Number of pages||6|
|Journal||Journal of International Dental and Medical Research|
|Publication status||Published - 2017|
- Aggressive periodontitis
- LPS A. actinomycetemcomitans