Immunohistochemical study of Curcuma xantorrhiza Roxb. and Morinda citirifolia L. ethanolic extract granules combination in high fat diet induced hyperlipidemia rats

Objectives: These studies were undertaken to assess the mechanism of Curcuma xanthorrhiza and Morinda citrifolia granules combination as anti-hyperlipidemic agent by immunohistochemical analysis in rats model.

Methods: The rats were given HFD by oral gavage and PTU in drink water for 10 days to induce hyperlipidemia. Then they were randomized and divided into six groups, negative control, simvastatin control, gemfibrozil control, and three treatment groups. The treatment group rats was treated with extract granules combination at 137; 411; 686 mg/kg body weight that administered orally for 28 days. By immunohistochemistry preparation, jejunum was scored for NPC1L1 expression, so do adipose tissue was score for LPL expression.

Results: Curcuma xanthorrhiza and Morinda citrifolia extract granules combination significantly (p<0.05) increased LPL expression at all dose (137; 411; 686 mg/kgBW). Curcuma xanthorrhiza and Morinda citrifolia extract granules combination also significantly (p<0.05) decreased NPC1L1 expression at dose 411 and 686 mg/kgBW, but not significant (p>0.05) at dose 137 mg/kgBW.

Conclusions: Curcuma xanthorrhiza and Morinda citrifolia extract granules combination can increase lipoprotein lipase expression, a rate limiting enzyme of plasma triglyceride removal and decrease NPC1L1 expression, an intestinal cholesterol absorption transporter.