Abstract
Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human. Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial. Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β. Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.
Original language | English |
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Pages (from-to) | 1333-1336 |
Number of pages | 4 |
Journal | Veterinary World |
Volume | 10 |
Issue number | 11 |
DOIs | |
Publication status | Published - 13 Nov 2017 |
Keywords
- Diagnostic kit
- Enzyme-linked immunosorbent assay
- Human
- Immunoglobulin G subclass
- Monoclonal antibody
- Toxocara cati
- Toxocariasis