Immunoglobulin subclass in experimental murine Toxocara cati infection

Setiawan Koesdarto; Sri Mumpuni; R. Heru Prasetyo

doi:10.14202/vetworld.2017.1333-1336

Immunoglobulin subclass in experimental murine Toxocara cati infection

Kusnoto, Setiawan Koesdarto, Sri Mumpuni, R. Heru Prasetyo

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human. Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial. Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β. Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

Original language	English
Pages (from-to)	1333-1336
Number of pages	4
Journal	Veterinary World
Volume	10
Issue number	11
DOIs	https://doi.org/10.14202/vetworld.2017.1333-1336
Publication status	Published - 13 Nov 2017

Keywords

Diagnostic kit
Enzyme-linked immunosorbent assay
Human
Immunoglobulin G subclass
Monoclonal antibody
Toxocara cati
Toxocariasis

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10.14202/vetworld.2017.1333-1336

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title = "Immunoglobulin subclass in experimental murine Toxocara cati infection",

abstract = "Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human. Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial. Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β. Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.",

keywords = "Diagnostic kit, Enzyme-linked immunosorbent assay, Human, Immunoglobulin G subclass, Monoclonal antibody, Toxocara cati, Toxocariasis",

author = "Kusnoto and Setiawan Koesdarto and Sri Mumpuni and Prasetyo, {R. Heru}",

note = "Funding Information: The study was supported by funding from the Directorate General of Higher Education (DIKTI) 2015, The National Education Ministry, Republic of Indonesia (018/SP2H/LT/DRPM/II/2015). Publisher Copyright: {\textcopyright} Kusnoto, et al.",

year = "2017",

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doi = "10.14202/vetworld.2017.1333-1336",

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T1 - Immunoglobulin subclass in experimental murine Toxocara cati infection

AU - Kusnoto,

AU - Koesdarto, Setiawan

AU - Mumpuni, Sri

AU - Prasetyo, R. Heru

N1 - Funding Information: The study was supported by funding from the Directorate General of Higher Education (DIKTI) 2015, The National Education Ministry, Republic of Indonesia (018/SP2H/LT/DRPM/II/2015). Publisher Copyright: © Kusnoto, et al.

PY - 2017/11/13

Y1 - 2017/11/13

N2 - Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human. Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial. Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β. Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

AB - Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human. Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial. Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β. Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

KW - Diagnostic kit

KW - Enzyme-linked immunosorbent assay

KW - Human

KW - Immunoglobulin G subclass

KW - Monoclonal antibody

KW - Toxocara cati

KW - Toxocariasis

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SN - 0972-8988

VL - 10

SP - 1333

EP - 1336

JO - Veterinary World

JF - Veterinary World

IS - 11

ER -

Immunoglobulin subclass in experimental murine Toxocara cati infection

Abstract

Keywords

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