TY - JOUR
T1 - Immunoglobulin AMG anti natural disaccharide octyl - Leprosy IDRI diagnostic (NDO-LID) serologic test for leprosy diagnosis
T2 - A pilot study
AU - Rumondor, Bayu Bijaksana
AU - Prakoeswa, Anisha Callista
AU - Trianita, Meva Nareza
AU - Iswahyudi,
AU - Herwanto, Nanny
AU - Listiawan, M. Yulianto
AU - Agusni, Indropo
AU - Izumi, Shinzo
AU - Duthie, Malcolm
AU - Prakoeswa, Cita Rosita Sigit
N1 - Funding Information:
The authors would like to thank Netherlands Leprosy Relief for supporting this project and also Malcolm Duthie (Infectious Disease Research Institute, Seattle, USA) for generous support of the antigen.
Publisher Copyright:
© 2019 Page Press Publications. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Leprosy remains a public health problem in Indonesia. Diagnostic tools have been developed in order to aid early diagnosis and prompt treatment. Phenolic glycolipid (PGL)-1 has been considered as a good candidate for leprosy diagnosis but has been found to have several limitations. More recently, the conjugation between natural disaccharide octyl (NDO) and leprosy IDRI diagnostic (LID)-1, known as NDO-LID, shows great promise because of its high specificity and sensitivity and its ability to detect leprosy before any clinical signs are present. The test incorporates the detection of IgM antibodies towards NDO and IgG antibodies towards LID-1. This study aimed to show the profile of IgM, IgG, and IgAMG antibody titers against NDO-LID to further discover its diagnostic potential. Sera from eight new leprosy patients from Surabaya, Indonesia were evaluated using ELISA detecting levels of IgG, IgM and IgAMG antibodies against NDO-LID antigen. Skin slit biopsy was also taken for smear and histopathology test. This study shows that the titer levels IgAMG anti NDO-LID were consistent with the results from smear and were consistently higher compared to IgM or IgG titer alone. IgAMG might have the potential to improve the sensitivity of NDO-LID serologic tests but further investigation is needed.
AB - Leprosy remains a public health problem in Indonesia. Diagnostic tools have been developed in order to aid early diagnosis and prompt treatment. Phenolic glycolipid (PGL)-1 has been considered as a good candidate for leprosy diagnosis but has been found to have several limitations. More recently, the conjugation between natural disaccharide octyl (NDO) and leprosy IDRI diagnostic (LID)-1, known as NDO-LID, shows great promise because of its high specificity and sensitivity and its ability to detect leprosy before any clinical signs are present. The test incorporates the detection of IgM antibodies towards NDO and IgG antibodies towards LID-1. This study aimed to show the profile of IgM, IgG, and IgAMG antibody titers against NDO-LID to further discover its diagnostic potential. Sera from eight new leprosy patients from Surabaya, Indonesia were evaluated using ELISA detecting levels of IgG, IgM and IgAMG antibodies against NDO-LID antigen. Skin slit biopsy was also taken for smear and histopathology test. This study shows that the titer levels IgAMG anti NDO-LID were consistent with the results from smear and were consistently higher compared to IgM or IgG titer alone. IgAMG might have the potential to improve the sensitivity of NDO-LID serologic tests but further investigation is needed.
KW - Immunoglobulin
KW - Leprosy
KW - NDO-LID
KW - Serologic test
UR - http://www.scopus.com/inward/record.url?scp=85071474940&partnerID=8YFLogxK
U2 - 10.4081/dr.2019.8025
DO - 10.4081/dr.2019.8025
M3 - Article
AN - SCOPUS:85071474940
SN - 2036-7392
VL - 11
JO - Dermatology Reports
JF - Dermatology Reports
IS - S1
M1 - 8025
ER -
