Immunogenicity evaluation of polimorphonuclear (PMN) cells, IL-2, IL-10 and IgG of biodegradable porous sponge cartilage scaffold (BPSCS), adipose derived mesenchymal stem cell (ADMSC) and secretome in New Zealand white rabbits with cartilage defect: In vivo experimental study

Brilliant Citra Wirashada, Dwikora Novembri Utomo, Purwati, Lukas Widhiyanto, Kukuh Dwiputra Hernugrahanto

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Abstract

Articular cartilage damage has limited healing potential based on its hypocellular and a vascularity nature. However, tissue engineering techniques through the use of scaffold and stem cells in the treatment of osteochondral defects has become one of the preferred therapeutic solutions. Also, bovine cartilage is one of the ingredients in making cheap and readily available scaffolds. There are various forms of the bovine scaffold, but, the Biodegradable Porous Sponge Bovine Cartilage Scaffold (BPSBCS) is a new form capable of inducing and facilitating better proliferation and differentiation of stem cells. However, there is need of more in vivo studies in finding out if the addition of secretomes and Adipose-Derived Mesenchymal Stem Cells (ADMSC) has the capacity to suppress the inflammatory response of sponge cartilage scaffold and cartilage defects in New Zealand white rabbits. 18 New Zealand white rabbits from the age of 6-9 months were divided into 3 groups, namely; the microfracture defect group with BPSCS implantation, the microfracture defect group with BPSCS and Secretome implantation, and the microfracture defect group with BPSCS and ADMSC implantation, in which each group was made up of 6 rabbits, adjusted for age, sex and weight. Rabbits were treated with full-thickness lesions on 4.5 cm2 to subchondral articular cartilage. The fibrin glue was implanted to fix BPSCS in the treatment groups. Then the acute inflammatory components such as IL-2, eosinophil, and neutrophil, chronic (IL-10), allergic reactions (IgG) and rejection reactions (IgG and basophil), were evaluated using ELISA and then histologically compared to each other. The observation of eosinophil, basophil, neutrophil, and PMN levels in the microfracture defect group with BPSCS on the first day were higher with p = 0.044. The observation of neutrophil and PMN levels in the microfracture defect group with BPSCS and ADMSC the third day was higher with p = 0.032. Then, observation of eosinophil, basophil, neutrophil, and PMN levels in the microfracture defect group with BPSCS and ADMSC were higher on the seventh day with p = 0.007. Furthermore, the observation of IL-2 levels with p = 0.399, in the microfracture defect group with BPSCS was higher than in the other 2 groups. Additionally, the observation of IL-10 levels in both blood preparations with p = 0.546, synovial with p = 0.115 and IgG levels with p = 0.077, in the microfracture defect group with BPSCS and ADMSC were higher than those in the other 2 groups. Biodegradable porous sponge cartilage scaffold has the capacity to synergize with Adipose-Derived Mesenchymal Stem Cell or secretome without any rejection and inflammatory effects after implantation in New Zealand White Rabbit cartilage defects.

Original languageEnglish
Pages (from-to)4811-4818
Number of pages8
JournalBiochemical and Cellular Archives
DOIs
Publication statusPublished - 1 Dec 2019

Keywords

  • Joint cartilage
  • Microfracture defect
  • Scaffold
  • Stem cell

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