Immunofluorescence assay method to detect dengue virus in Paniai-Papua

Teguh Hari Sucipto, Nur Laila Fitriati Ahwanah, Siti Churrotin, Norifumi Matake, Tomohiro Kotaki, Soegeng Soegijanto

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

The dengue viruses (DENV), which include in the family Flaviviridae and the genus Flavivirus, was endemic in tropical areas and had been transmitted to humans by Aedes aegypti. An increasing number of immigrants from endemic areas to the non-endemic areas have emphasized the need for a simple and reliable test for the diagnosis of dengue virus infection. The purpose of this study was to detect the dengue virus by immunofluorescence assay (IFA) in the general population at Paniai-Papua. The results obtained from this study had showed a significantly better discrimination for DENV specific IgG antibodies. A total of 158 samples, 116 samples were IgG antibodies positive and 42 samples were negative. The conclusion of this study, Papua is not only a malaria endemic area, but also dengue virus infections were detected by IFA method. Therefore, the IFA can be used as an important diagnostic tool, which is a quick and an easy way to test samples from immigrants who come to the non-endemic areas.

Original languageEnglish
Title of host publication5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015
EditorsMoh. Yasin, Sulaiman W. Harun
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735413641
DOIs
Publication statusPublished - 15 Mar 2016
Event5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015 - Surabaya, Indonesia
Duration: 16 Oct 201517 Oct 2015

Publication series

NameAIP Conference Proceedings
Volume1718
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015
Country/TerritoryIndonesia
CitySurabaya
Period16/10/1517/10/15

Keywords

  • DENV
  • General Population
  • Immunofluoroscence Assay
  • Paniai-Papua

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