Identifikasi Molekuler Blastocystis sp. pada Monyet Ekor Panjang (Macaca fascicularis) di Taman Nasional Baluran, Situbondo, Jawa Timur

The close contact between macaque and humans can increase the risk of disease transmissions. Blastocystis sp. is a gastrointestinal protozoa in human and animal which considered zoonotic. This study aimed to identify Blastocystis sp. infecting macaque with molecular identification. To provide identification about Blastocystis sp., we adopted both morphologic and molecular methods. We collected 90 unidentified individuals of long tailed macaque in Baluran National Park and did microscopic screening after culture in Jones Medium. A total of 28 from positive microscopic samples were used in polymerase chain reaction targeting barcode region with visualization of 600bp. Three samples with positive band 600bp continued sequencing. The result processed in BLAST and MLST. One sample confirmed as Blastocystis sp. with mix infection of subtype 1 allele 2 and subtype 3 allele 34. The results indicate that Blastocystis sp. existed in macaques in Baluran National Park with low prevalence.