Identification of secondary metabolites and activity test of Ganoderma lucidum methanol extract as anti-termite (Coptotermes curvignathus) biopesticide

Ganoderma lucidum is commonly known as medicinal fungi used to treat various disease. Its containing bioactive compounds are still widely developed and examined for discovery of new medicine. However, few studies had conducted on the application of G. lucidum as pesticide, especially as anti-termite (Coptotermes curvignathus). This study was conducted to determine secondary metabolites of Lingzhi fungi using thin layer chromatography and its potential as anti-termite (Coptotermes curvignathus) biopesticide. Methods used on this study including extraction which used methanol for maceration, identification of secondary metabolites using thin layer chromatography, toxicity test of Lingzhi methanol extract on soil termites at concentration of 5%, 10%, 20%, 30%, and 40% for 3 days duration. Anti-termite toxicity test was evaluated based on the number of termite mortality and termite repellant test. Result showed that methanol extract of Ganoderma lucidum (Lingzhi) contained terpenoid and alkaloid. Optimal concentration resulting in the most mortality of termites was found to be 40% (100%termite mortality and 82% repellant). This indicated that Lingzhi methanol extract had potential as anti-termite (Coptotermes curvignathus) natural biopesticide.