TY - JOUR
T1 - Identification of non-typhoidal salmonella from diarrheal pediatric patients in Surabaya, Indonesia
AU - Juniastuti,
AU - Athiyyah, Alpha Fardah
AU - Darma, Andy
AU - Yamani, Laura Navika
AU - Ferandra, Virginia Ayu
AU - Fajar, Nur Syamsiyatul
AU - Hariyono, Sugeng
AU - Amin, Mochamad
AU - Utsumi, Takako
AU - Ranuh, Reza
AU - Sudarmo, Subijanto M.
AU - Lusida, Maria Inge
N1 - Funding Information:
The authors thank all participants and GC Pandango, AFW Putri, LP Dewi, VV Pasaribu, and RA Satjadibrata, Dr Soetomo General Hospital, Surabaya for their kindness in collecting specimens. The study was supported by a grant from Airlangga Health Science Institute, Universitas Airlangga.
Publisher Copyright:
© 2019, SEAMEO TROPMED Network. All rights reserved.
PY - 2019/3
Y1 - 2019/3
N2 - Salmonella Typhi and Salmonella Paratyphi are the predominant agents of diarrheal disease. However, non-typhoidal Salmonella (NTS) serovars are becoming increasingly global. Indonesia is endemic for typhoid fever, but data on NTS is limited. The study employed multiplex PCR (mPCR) to identify NTS in diarrheal children. Bacterial cultures grown on Salmonella Shigella agar from 80 fecal samples of diarrheal pediatric patients in Dr Soetomo General Hospital, Surabaya, Indonesia were characterized by mPCR as NTS Enteritidis, Infantis, Thompson, and Typhimurium. Confirmation by direct amplicon sequencing and by conventional biochemical and serological tests revealed only one NTS as Infantis and one isolate each of S. Paratyphi A, S. Paratyphi C and S. Typhi. The clinical manifestation of S. Infantis infection was milder than that of S. Paratyphi or S. Typhi infection. Thus confirmation tests should be conducted to confirm NTS identification by mPCR.
AB - Salmonella Typhi and Salmonella Paratyphi are the predominant agents of diarrheal disease. However, non-typhoidal Salmonella (NTS) serovars are becoming increasingly global. Indonesia is endemic for typhoid fever, but data on NTS is limited. The study employed multiplex PCR (mPCR) to identify NTS in diarrheal children. Bacterial cultures grown on Salmonella Shigella agar from 80 fecal samples of diarrheal pediatric patients in Dr Soetomo General Hospital, Surabaya, Indonesia were characterized by mPCR as NTS Enteritidis, Infantis, Thompson, and Typhimurium. Confirmation by direct amplicon sequencing and by conventional biochemical and serological tests revealed only one NTS as Infantis and one isolate each of S. Paratyphi A, S. Paratyphi C and S. Typhi. The clinical manifestation of S. Infantis infection was milder than that of S. Paratyphi or S. Typhi infection. Thus confirmation tests should be conducted to confirm NTS identification by mPCR.
KW - Diarrhea
KW - Indonesia
KW - Non-typhoidal Salmonella
KW - Pediatric patient
UR - http://www.scopus.com/inward/record.url?scp=85109521493&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85109521493
SN - 0125-1562
VL - 50
SP - 258
EP - 265
JO - Southeast Asian Journal of Tropical Medicine and Public Health
JF - Southeast Asian Journal of Tropical Medicine and Public Health
IS - 2
ER -
