Identification of mycobacterium tuberculosis in sputum smear slide using automatic scanning microscope

Riries Rulaningtyas, Andriyan B. Suksmono, Tati L.R. Mengko, Putri Saptawati

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

6 Citations (Scopus)


Sputum smear observation has an important role in tuberculosis (TB) disease diagnosis, because it needs accurate identification to avoid high errors diagnosis. In development countries, sputum smear slide observation is commonly done with conventional light microscope from Ziehl-Neelsen stained tissue and it doesn't need high cost to maintain the microscope. The clinicians do manual screening process for sputum smear slide which is time consuming and needs highly training to detect the presence of TB bacilli (mycobacterium tuberculosis) accurately, especially for negative slide and slide with less number of TB bacilli. For helping the clinicians, we propose automatic scanning microscope with automatic identification of TB bacilli. The designed system modified the field movement of light microscope with stepper motor which was controlled by microcontroller. Every sputum smear field was captured by camera. After that some image processing techniques were done for the sputum smear images. The color threshold was used for background subtraction with hue canal in HSV color space. Sobel edge detection algorithm was used for TB bacilli image segmentation. We used feature extraction based on shape for bacilli analyzing and then neural network classified TB bacilli or not. The results indicated identification of TB bacilli that we have done worked well and detected TB bacilli accurately in sputum smear slide with normal staining, but not worked well in over staining and less staining tissue slide. However, overall the designed system can help the clinicians in sputum smear observation becomes more easily.

Original languageEnglish
Title of host publication5th Asian Physics Symposium, APS 2012
EditorsSparisoma Viridi, Khairul Basar, Ferry Iskandar, Bobby Eka Gunara, Wahyu Srigutomo
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735412989
Publication statusPublished - 16 Apr 2015
Event5th Asian Physics Symposium, APS 2012 - Bandung, Indonesia
Duration: 10 Jul 201212 Jul 2012

Publication series

NameAIP Conference Proceedings
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616


Conference5th Asian Physics Symposium, APS 2012


  • Automatic scanning microscope
  • Classification
  • Feature extraction
  • Image processing
  • Tuberculosis
  • Ziehl-Neelsen


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