TY - JOUR
T1 - Identification and isolation of japanese encephalitis virus genotype IV from culex vishnui collected in Bali, Indonesia in 2019
AU - Faizah, Astri Nur
AU - Kobayashi, Daisuke
AU - Maekawa, Yoshihide
AU - Amoa-Bosompem, Michael
AU - Fauziyah, Shifa
AU - Mulyatno, Kris Cahyo
AU - Subekti, Sri
AU - Rohmah, Etik Ainun
AU - Lusida, Maria Inge
AU - Mori, Yasuko
AU - Miura, Kozue
AU - Hirayama, Kazuhiro
AU - Isawa, Haruhiko
AU - Sawabe, Kyoko
N1 - Publisher Copyright:
Copyright © 2021 by The American Society of Tropical Medicine and Hygiene
PY - 2021/9
Y1 - 2021/9
N2 - Japanese encephalitis virus (JEV) is transmitted between swine, migratory birds, and Culex mosquitoes, and has circulated indigenously in Asia for almost a century. Despite being the country with the highest JEV diversity, surveillance targeting of Indonesia’s vectors is scarce. This study collected mosquitoes from several locations in Tabanan Regency, Bali Island, Indonesia. We captured and classified 3,032 adult Culex mosquitoes into seven species, with Culex vishnui subgroup mosquitoes making up approximately 90% of the total. Japanese encephalitis virus was identified by next-generation sequencing (NGS) analysis of a Cx. vishnui mosquito pool. Genetic and phylogenetic analysis revealed the JEV as genotype (G) IV. The nucleotide identity was 99% with other JEV GIV isolates obtained from swine sera in 2017 on Bali Island and from a human patient in Australia with a travel history to Bali in 2019. This finding indicated that JEV GIV persists in restricted areas and is circulating between swine-mosquito vectors.
AB - Japanese encephalitis virus (JEV) is transmitted between swine, migratory birds, and Culex mosquitoes, and has circulated indigenously in Asia for almost a century. Despite being the country with the highest JEV diversity, surveillance targeting of Indonesia’s vectors is scarce. This study collected mosquitoes from several locations in Tabanan Regency, Bali Island, Indonesia. We captured and classified 3,032 adult Culex mosquitoes into seven species, with Culex vishnui subgroup mosquitoes making up approximately 90% of the total. Japanese encephalitis virus was identified by next-generation sequencing (NGS) analysis of a Cx. vishnui mosquito pool. Genetic and phylogenetic analysis revealed the JEV as genotype (G) IV. The nucleotide identity was 99% with other JEV GIV isolates obtained from swine sera in 2017 on Bali Island and from a human patient in Australia with a travel history to Bali in 2019. This finding indicated that JEV GIV persists in restricted areas and is circulating between swine-mosquito vectors.
UR - http://www.scopus.com/inward/record.url?scp=85115325222&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.20-1554
DO - 10.4269/ajtmh.20-1554
M3 - Article
C2 - 34280147
AN - SCOPUS:85115325222
SN - 0002-9637
VL - 105
SP - 813
EP - 817
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 3
ER -
