Identification and In Silico Characterization of a Conserved Peptide on Influenza Hemagglutinin Protein: A New Potential Antigen for Universal Influenza Vaccine Development

Atin Khalaj-Hedayati, Seyedehmaryam Moosavi, Otilia Manta, Mohamed H. Helal, Mohamed M. Ibrahim, Zeinhom M. El-Bahy, Ganden Supriyanto

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Antigenic changes in surface proteins of the influenza virus may cause the emergence of new variants that necessitate the reformulation of influenza vaccines every year. Universal influenza vaccine that relies on conserved regions can potentially be effective against all strains regardless of any antigenic changes and as a result, it can bring enormous public health impact and economic benefit worldwide. Here, a conserved peptide (HA288–107) on the stalk domain of hemagglutinin glycoprotein is identified among highly pathogenic influenza viruses. Five top-ranked B-cell and twelve T-cell epitopes were recognized by epitope mapping approaches and the corresponding Human Leukocyte Antigen alleles to T-cell epitopes showed high population coverage (>99%) worldwide. Moreover, molecular docking analysis indicated that VLMENERTL and WTYNAELLV epitopes have high binding affinity to the antigen-binding groove of the HLA-A*02:01 and HLA-A*68:02 molecules, respectively. Theoretical physicochemical properties of the peptide were assessed to ensure its thermostability and hydrophilicity. The results suggest that the HA288–107 peptide can be a promising antigen for universal influenza vaccine design. However, in vitro and in vivo analyses are needed to support and evaluate the effectiveness of the peptide as an immunogen for vaccine development.

Original languageEnglish
Article number2796
JournalNanomaterials
Volume13
Issue number20
DOIs
Publication statusPublished - Oct 2023

Keywords

  • epitope mapping
  • hemagglutinin
  • immunoinformatic
  • nanoparticle
  • peptide-based vaccine
  • universal influenza vaccine

Fingerprint

Dive into the research topics of 'Identification and In Silico Characterization of a Conserved Peptide on Influenza Hemagglutinin Protein: A New Potential Antigen for Universal Influenza Vaccine Development'. Together they form a unique fingerprint.

Cite this