Identification, characterization, and purification of xylanase pc-01 isolated from pacet hot spring

Mamik Damayanti; Ni Nyoman Purwani; Ni Made Dwi Purwadi; Lailatul Fithri

Identification, characterization, and purification of xylanase pc-01 isolated from pacet hot spring

Mamik Damayanti, Ni Nyoman Purwani, Ni Made Dwi Purwadi, Lailatul Fithri

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

This research aims to characterize, identify and purify the xylanolytic enzymes by hydrophobic interaction and anion exchange chromatography via the endo-β-xylanase activity test with the DNS method. The best results obtained from anion-exchange chromatography of third fraction pH 5 with the purity level of 78.25 times higher than crude extract. In conclusion, Characteristics of bacterial isolates PC-01 are rod-shaped bacterial cells, Gram-positive, and have subterminal oval endospores.

Original language	English
Pages (from-to)	1074-1091
Number of pages	18
Journal	Opcion
Volume	35
Issue number	SpecialEdition24
Publication status	Published - 2019

Keywords

Chromatography
Endo-Β-Xylanase
Hydrophobic
Interactions

Cite this

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title = "Identification, characterization, and purification of xylanase pc-01 isolated from pacet hot spring",

abstract = "This research aims to characterize, identify and purify the xylanolytic enzymes by hydrophobic interaction and anion exchange chromatography via the endo-β-xylanase activity test with the DNS method. The best results obtained from anion-exchange chromatography of third fraction pH 5 with the purity level of 78.25 times higher than crude extract. In conclusion, Characteristics of bacterial isolates PC-01 are rod-shaped bacterial cells, Gram-positive, and have subterminal oval endospores.",

keywords = "Chromatography, Endo-Β-Xylanase, Hydrophobic, Interactions",

author = "Mamik Damayanti and Purwani, {Ni Nyoman} and Purwadi, {Ni Made Dwi} and Lailatul Fithri",

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AU - Damayanti, Mamik

AU - Purwani, Ni Nyoman

AU - Purwadi, Ni Made Dwi

AU - Fithri, Lailatul

PY - 2019

Y1 - 2019

N2 - This research aims to characterize, identify and purify the xylanolytic enzymes by hydrophobic interaction and anion exchange chromatography via the endo-β-xylanase activity test with the DNS method. The best results obtained from anion-exchange chromatography of third fraction pH 5 with the purity level of 78.25 times higher than crude extract. In conclusion, Characteristics of bacterial isolates PC-01 are rod-shaped bacterial cells, Gram-positive, and have subterminal oval endospores.

AB - This research aims to characterize, identify and purify the xylanolytic enzymes by hydrophobic interaction and anion exchange chromatography via the endo-β-xylanase activity test with the DNS method. The best results obtained from anion-exchange chromatography of third fraction pH 5 with the purity level of 78.25 times higher than crude extract. In conclusion, Characteristics of bacterial isolates PC-01 are rod-shaped bacterial cells, Gram-positive, and have subterminal oval endospores.

KW - Chromatography

KW - Endo-Β-Xylanase

KW - Hydrophobic

KW - Interactions

UR - http://www.scopus.com/inward/record.url?scp=85077626198&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85077626198

SN - 1012-1587

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JO - Opcion

JF - Opcion

IS - SpecialEdition24

ER -

Identification, characterization, and purification of xylanase pc-01 isolated from pacet hot spring

Abstract

Keywords

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