TY - JOUR
T1 - Hypoxic preconditioning effect on stromal cells derived factor-1 and C-X-C chemokine receptor type 4 expression in Wistar rat's (Rattus norvegicus) bone marrow mesenchymal stem cells (in vitro study)
AU - Mulyani, Sri Wigati Mardi
AU - Ernawati, Diah Savitri
AU - Astuti, Eha Renwi
AU - Rantam, Fedik Abdul
N1 - Funding Information:
The authors would like to thank the Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. This laboratory work was supported by grants from DIPA BO. The authors would like to thank Universitas Airlangga and Stem Cell and Tissue Bank Center RSUD Dr. Soetomo Surabaya, Indonesia. The research grant is funded by Penelitian Unggulan Perguruan Tinggi (PUPT) 2017 DIPA DRPM Research, Technology and Higher Education Ministry of Indonesia. Letter of Appointment Agreement of Research Program number: 137, PUPT, thp-I/2017; 586, UN3/2017 May 12, 2017.
Publisher Copyright:
© Mulyani, et al.
PY - 2018/7/19
Y1 - 2018/7/19
N2 - Aim: To examine the effect of hypoxic preconditions on the ability of bone marrow stem cells culture mediated expression C-X-C chemokine receptor type 4 (CXCR4) and stromal cells derived factor-1 (SDF-1) in vitro. Materials and Methods: Bone marrow mesenchymal stem cells (BMSCs) were derived from 12 femurs of 200 g Wistar male rats. The animals were euthanized before BMSCs isolation. BMSCs were divided into two groups, control group: Normoxic condition 21% O2 and treatment group: Hypoxic condition 1% O2. The characterization of BMSCs was analyzed using flow cytometry by cluster differentiation 34 and cluster differentiation 105. The expression of CXCR4 and SDF-1 measured using immunocytochemistry immunofluorescence label after 48-h incubation in a low-tension oxygen chamber with an internal atmosphere consisting of 95% N2, 5% CO2, and 1% O2. All data were subjected to a normality test and then analyzed using t-test statistic (p < 0.05). Results: The characterization of bone marrow stem cells showed positive cluster differentiation 34 and cluster differentiation 105. A hypoxic precondition (1% O2) in culture increases CXCR4 (p=0.000) and SDF-1 expression than normoxic conditions (p=0.000) (p < 0.05). Conclusion: Hypoxic preconditioning with 1% O2 increase CXCR4 and SDF1 expression.
AB - Aim: To examine the effect of hypoxic preconditions on the ability of bone marrow stem cells culture mediated expression C-X-C chemokine receptor type 4 (CXCR4) and stromal cells derived factor-1 (SDF-1) in vitro. Materials and Methods: Bone marrow mesenchymal stem cells (BMSCs) were derived from 12 femurs of 200 g Wistar male rats. The animals were euthanized before BMSCs isolation. BMSCs were divided into two groups, control group: Normoxic condition 21% O2 and treatment group: Hypoxic condition 1% O2. The characterization of BMSCs was analyzed using flow cytometry by cluster differentiation 34 and cluster differentiation 105. The expression of CXCR4 and SDF-1 measured using immunocytochemistry immunofluorescence label after 48-h incubation in a low-tension oxygen chamber with an internal atmosphere consisting of 95% N2, 5% CO2, and 1% O2. All data were subjected to a normality test and then analyzed using t-test statistic (p < 0.05). Results: The characterization of bone marrow stem cells showed positive cluster differentiation 34 and cluster differentiation 105. A hypoxic precondition (1% O2) in culture increases CXCR4 (p=0.000) and SDF-1 expression than normoxic conditions (p=0.000) (p < 0.05). Conclusion: Hypoxic preconditioning with 1% O2 increase CXCR4 and SDF1 expression.
KW - Bone marrow stem cells
KW - C-X-C chemokine receptor type 4
KW - Hypoxic preconditioning
KW - Mesenchymal stem cells
KW - Stromal cells derived factor-1
UR - http://www.scopus.com/inward/record.url?scp=85050311196&partnerID=8YFLogxK
U2 - 10.14202/vetworld.2018.965-970
DO - 10.14202/vetworld.2018.965-970
M3 - Article
AN - SCOPUS:85050311196
SN - 0972-8988
VL - 11
SP - 965
EP - 970
JO - Veterinary World
JF - Veterinary World
IS - 7
ER -