Hyperbaric Oxygen Therapy to Minimize Orthodontic Relapse in Rabbits

Yun Mukmin Akbar, Ani Melani Maskoen, Endah Mardiati, Ganesha Wandawa, Amaliya Amaliya, Ida Bagus Narmada, Nina Djustiana, Ida Ayu Evangelina, Rasmi Rikmasari, Mas Rizky Anggun

Research output: Contribution to journalArticlepeer-review


Objectives The purpose of the present study was to discover how hyperbaric oxygen therapy (HBOT) could reduce orthodontic relapse by altering the expressions of hypoxia-inducible factor (HIF)-1 messenger ribonucleic acid (mRNA), type I collagen (Col I), and matrix metalloproteinase-1 (MMP-1) in the gingival supracrestal fibers in rabbits. Materials and Methods This study involved 44 male rabbits (Oryctolagus cuniculus) randomly divided into the normal group (K0), the orthodontic group without HBOT (K1), and the orthodontic group with HBOT (K2). Following orthodontic separation of the two upper central incisors, a retention phase and relapse assessment were performed. The HBOT was performed for a period of 2, 4, 6, 8, and 10 days after retention. HIF-1α transcription was assessed employing real-time polymerase chain reaction, whereas Col I and MMP-1 proteins were examined using immunohistochemistry. The orthodontic relapse was measured clinically using a digital caliper. Statistical Analysis We used the one-way analysis of variance followed by Tukey's post hoc for multiple comparisons to measure differences between pairs of means; a p- value of 0.05 was considered statistically significant. Results HBOT significantly increased the HIF-1α mRNA expression (p = 0.0140), increased Col I (p = 0.0043) and MMP-1 (p = 0.0068) on the tensioned and pressured side of the gingival supracrestal fibers, respectively, and clinically decreased the relapse (p = 3.75 × 10 -40). Conclusion HBOT minimizes orthodontic relapse by influencing HIF-1α expression, collagen synthesis (Col I), and degradation (MMP-1). This result suggests that HBOT has the potential to be used as an adjunctive method in the orthodontic retention phase.

Original languageEnglish
JournalEuropean Journal of Dentistry
Publication statusAccepted/In press - 2024


  • animal model
  • collagen synthesis and degradation
  • hyperbaric oxygen therapy
  • orthodontic relapse
  • supracrestal gingival fibers


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