Objective: To evaluate the effect of hyperbaric oxygen (HBO) therapy on switching phenotypes from M1 macrophage cluster differentiation (CD) 80 to M2 (CD163) through changes in expression of hypoxia-inducible factor-1α (HIF-1α) in antigen and collagen-induced arthritis (ACIA). Methods: We used male Balb / C mice as models of ACIA. The severity of arthritis was assessed by a clinical arthritis score and with the diameter of the left knee joint measured using a calliper. Interleukine-10 (IL-10) level in plasma was measured by ELISA technique. Expression of HIF-1α, CD80 dan CD163 were measured by immunochemistry technique. We used IBM SPSS statistics version 22.0. Statistical significance test was done by independent t-test. Results: The level of expression of HIF-1α decreased significantly p = 0.000 (p < 0.05) in the HBO group (33.20 ± 10.12) compared to non-HBO group (106.40 ± 21.20). The level of expression of CD80 decreased significantly p = 0.000 (p < 0.05) in the HBO group (69.80 ± 6.71) compared to non-HBO group ((308.10 ± 25.01). The level of expression of CD163 increased significantly p = 0.000 (p < 0.05) in the HBO group (153.00 ± 8.78) compared to non-HBO group (44.90 ± 2.89). The clinical arthritis score and diameter of the left knee joint of mice decreased significantly (p < 0.05) and IL-10 levels increased significantly (p < 0.05) in the HBO group compared to the non-HBO group. Conclusion: HBO has an effect on switching the M1 phenotypes to M2 through a decrease in the expression of HIF-1α while increasing IL-10 production thereby reducing arthritis in ACIA. Therefore, HBO has therapeutic potential for the treatment of rheumatoid arthritis.
|Number of pages||4|
|Journal||International Medical Journal|
|Publication status||Published - Dec 2019|