Hydrolysis of imperata cylindrica (L.) Beauv. By penicillium sp., aspergillus Niger and trichoderma viride as bioethanol basic ingredient production

This study aims to determine the effect of pretreatment on the content of cellulose, hemicellulose, lignin, and combinations types of mold (Penicillium sp., AspergillusNiger and Trichodermaviride) and the incubation time (1, 2, 3, 4, 5, 6, 7, 8 days) against dry biomass, pH value, enzyme activity value (filter paper-ase (FP-ase), endoglucanase (EG), and cellobiohydrolase (CBH). The result show that delignification reached by 2% NaOH pretreated substrate andincrease the surface area of cellulose on cogongrass substrates with cellulose level48.32%, hemicellulose level 9.88% andlignin level1.14%.The fibers of the sample distorted as figured using SEM (Scanning Electronic Microscope). The microfibril section was torn and completely exposed, thus increased the external surface area and porosity. Compared with two other molds, Aspergillus Niger had optimum cellulase enzyme activity achieved on the 7^th day of incubation time with filter paper-ase enzyme value of 3.97 U/ml, endoglucanase (EG) enzyme value of 4.53 U/ml and cellobiohydrolase (CBH) enzyme value of 4.45 U/ml. Imperata cylindrica (L.) Beauv.,can be used as substrate for cellulase enzyme production by Aspergillus Niger as the basic ingredient of bioethanol.