Hyaluronic Acid (HA)-Methylcellulose (MC)-based hydrogel with antibacterial AgNPs as a post-surgery intraperitoneal anti-adhesive physical barrier

Post-surgery Peritoneal adhesion is a common problem causing complication after abdominal surgery, even death. This study aims to prove the hydrogel potential and obtain the optimum composition of AgNPs as intraperitoneal anti-adhesive physical barrier. The synthesize started with mixture of HA-MC from freeze-dry process with variation of AgNPs of 0, 1, 2, 3, and 5 ppm in Phosphate Buffer Saline (PBS). The result of Fourier Transform Infrared (FTIR) showed the crosslinking bonding in samples showed by the new imine bond on 1556.55 cm^-1 and 1548.84 cm^-1 on 0 and 3 ppm of AgNPs concentration. The composition of optimum AgNPs as HA-MC-AgNPs hydrogel composition was the sample with 3 ppm with degradation rate of 90% on the 11^th day which is approaching the ideal value of intraperitoneal antiadhesive physical barrier degradation rate and the cell viability of 59.45% which showed a nontoxic property. It also has high sensitivity to bacteria with clear zone diameter of 23.5 mm and swelling ratio which meets the standard and good mechanical properties. The HA-MC-AgNPs composite is safe and has potential to be applied as intraperitoneal antiadhesive physical barrier hydrogel based on the functional groups, degradation rate, cell viability, antibacterial test, mechanical test and swelling test.