Human Umbilical Cord Mesenchymal Stem Cells attachment in the Hydroxyapatite-Tricalcium Phosphate Scaffold in vitro

Bone grafts in prosthodontics is required, particularly for socket preservation during denture fabrication. The hydroxyapatite-tricalcium phosphate (HA-TCP) scaffold is one of the materials that may be utilized to preserve sockets because it is employed as a bone regeneration substitute material. This scaffold has the features of being biocompatible, degradable, and non-toxic. To achieve biocompatibility, the scaffold must be readily linked to cells containing growth factors. Human Umbilical Cord Mesenchymal Stem Cells were used to seed the scaffold (HUCMSCs). In bone defect instances, a combination of HA-TCP scaffold material and hUCMSCs is likely to be used as bone remodeling treatment for socket preservation. Objective: this study aimed to investigate the attachment of hUCMSCs in the HA-TCP scaffold using a confocal microscope. The scaffold HA-TCP samples were separated into four groups, and the control group was divided into two, namely HA-TCP 24 hours and HA-TCP 72 hours. The treatment group's sample was separated into two seeding times, 24 hours (P1) and 72 hours (P2) (P2). The sample was subsequently treated with bromodeoxyuridine (BrDU), and the quantity of hUCMSCs attachment was measured using a 400x magnification confocal lightning scanning microscope (CLSM). The Man-Whitney test was used with Kruskal-Wallis analysis to determine the difference between groups (p<0.05). After seeding for 24 hours, hUCMSCS may be attached to the HA-TCP scaffold with a significant difference between the groups. There is a substantial difference between the treatment and control groups, with 24 hours being the optimal shedding period.