Human chorionic gonadotropin from urine of pregnant women for in vitro maturation of madura cattle oocytes

Herry Agoes Hermadi; Raden Tatang Santanu Adikara; Masud Hariadi; Erma Safitri

Human chorionic gonadotropin from urine of pregnant women for in vitro maturation of madura cattle oocytes

Herry Agoes Hermadi, Raden Tatang Santanu Adikara, Masud Hariadi, Erma Safitri

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The purpose of this study was to test whether human chorionic gonadotropin (hCG) from urine of pregnant women can be used for in vitro maturation of Madura cattle oocytes. Urine samples were collected from 50 healthy pregnant women gestating for 1.5 to 3.5 months. Molecular weights of hCG were 37 kDa and 22 kDa. HCG levels measured via ELISA had 10 times the deposition, with an average of 27,333 mIU/l at 1.5 months and 105,667 mIU/l at 3.5 months, respectively. T-test showed no significant difference in oocyte maturation rate between the control group with patent hCG and hCG from pregnant women at P>0.05. This study illustrates that hCG from pregnant women at 1.5 to 3.5 months can aid in in vitro maturationof Madura cattle oocytes.

Original language	English
Pages (from-to)	127-132
Number of pages	6
Journal	Philippine Journal of Veterinary Medicine
Volume	55
Issue number	Special Issue
Publication status	Published - 1 Dec 2018

Keywords

HCG
In vitro maturation
Madura beef cattle
Oocytes
Urine of pregnant women

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Human chorionic gonadotropin from urine of pregnant women for in vitro maturation of madura cattle oocytes",

abstract = "The purpose of this study was to test whether human chorionic gonadotropin (hCG) from urine of pregnant women can be used for in vitro maturation of Madura cattle oocytes. Urine samples were collected from 50 healthy pregnant women gestating for 1.5 to 3.5 months. Molecular weights of hCG were 37 kDa and 22 kDa. HCG levels measured via ELISA had 10 times the deposition, with an average of 27,333 mIU/l at 1.5 months and 105,667 mIU/l at 3.5 months, respectively. T-test showed no significant difference in oocyte maturation rate between the control group with patent hCG and hCG from pregnant women at P>0.05. This study illustrates that hCG from pregnant women at 1.5 to 3.5 months can aid in in vitro maturationof Madura cattle oocytes.",

keywords = "HCG, In vitro maturation, Madura beef cattle, Oocytes, Urine of pregnant women",

author = "Hermadi, {Herry Agoes} and Adikara, {Raden Tatang Santanu} and Masud Hariadi and Erma Safitri",

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journal = "Philippine Journal of Veterinary Medicine",

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TY - JOUR

T1 - Human chorionic gonadotropin from urine of pregnant women for in vitro maturation of madura cattle oocytes

AU - Hermadi, Herry Agoes

AU - Adikara, Raden Tatang Santanu

AU - Hariadi, Masud

AU - Safitri, Erma

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The purpose of this study was to test whether human chorionic gonadotropin (hCG) from urine of pregnant women can be used for in vitro maturation of Madura cattle oocytes. Urine samples were collected from 50 healthy pregnant women gestating for 1.5 to 3.5 months. Molecular weights of hCG were 37 kDa and 22 kDa. HCG levels measured via ELISA had 10 times the deposition, with an average of 27,333 mIU/l at 1.5 months and 105,667 mIU/l at 3.5 months, respectively. T-test showed no significant difference in oocyte maturation rate between the control group with patent hCG and hCG from pregnant women at P>0.05. This study illustrates that hCG from pregnant women at 1.5 to 3.5 months can aid in in vitro maturationof Madura cattle oocytes.

AB - The purpose of this study was to test whether human chorionic gonadotropin (hCG) from urine of pregnant women can be used for in vitro maturation of Madura cattle oocytes. Urine samples were collected from 50 healthy pregnant women gestating for 1.5 to 3.5 months. Molecular weights of hCG were 37 kDa and 22 kDa. HCG levels measured via ELISA had 10 times the deposition, with an average of 27,333 mIU/l at 1.5 months and 105,667 mIU/l at 3.5 months, respectively. T-test showed no significant difference in oocyte maturation rate between the control group with patent hCG and hCG from pregnant women at P>0.05. This study illustrates that hCG from pregnant women at 1.5 to 3.5 months can aid in in vitro maturationof Madura cattle oocytes.

KW - HCG

KW - In vitro maturation

KW - Madura beef cattle

KW - Oocytes

KW - Urine of pregnant women

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M3 - Article

AN - SCOPUS:85056852570

SN - 0031-7705

VL - 55

SP - 127

EP - 132

JO - Philippine Journal of Veterinary Medicine

JF - Philippine Journal of Veterinary Medicine

IS - Special Issue

ER -

Human chorionic gonadotropin from urine of pregnant women for in vitro maturation of madura cattle oocytes

Abstract

Keywords

UN SDGs

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