Human age estimation through DNA metilation analysis method from bite mark samples in forensic identification

Ahmad Yudianto; A. R. Mieke Sylvia Margaretta; Fery Setiawan; Ma’Rifatul Ula

doi:10.37506/ijfmt.v15i1.13500

Human age estimation through DNA metilation analysis method from bite mark samples in forensic identification

Ahmad Yudianto, A. R. Mieke Sylvia Margaretta, Fery Setiawan, Ma’Rifatul Ula

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Age estimation are often calculated using bone and teeth samples, but are limited to human skeleton findings. The aim of this research is to estimate human age through DNA methylation method from bite mark samples. Result: There were 40 bite mark samples obtained from healthy volunteers. DNA samples were isolated using DNAzol reagent and converted using DNA methylation kit. The isolated DNA were amplified and electrophoreses was conducted using agarose gel. Electrophoresis result was used as length reference for the sequenced band and analyzed for methylation percentage and correlation with age estimation. Statistical test showed that there was a significant correlation between DNA methylation percentage with age estimation both in men (r Pearson 0.767) and women (r Pearson 0.878). Conclusion: Cpgplot emboss analysis for DNA methylation mean percentage in men tend to increase in accordance to age categories, whereas for women, the mean DNA methylation percentage in age categories was stable or constant.

Original language	English
Pages (from-to)	706-713
Number of pages	8
Journal	Indian Journal of Forensic Medicine and Toxicology
Volume	15
Issue number	1
DOIs	https://doi.org/10.37506/ijfmt.v15i1.13500
Publication status	Published - 1 Jan 2021
Externally published	Yes

Keywords

Age estimation
Bite mark
DNA methylation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.37506/ijfmt.v15i1.13500

Cite this

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title = "Human age estimation through DNA metilation analysis method from bite mark samples in forensic identification",

abstract = "Background: Age estimation are often calculated using bone and teeth samples, but are limited to human skeleton findings. The aim of this research is to estimate human age through DNA methylation method from bite mark samples. Result: There were 40 bite mark samples obtained from healthy volunteers. DNA samples were isolated using DNAzol reagent and converted using DNA methylation kit. The isolated DNA were amplified and electrophoreses was conducted using agarose gel. Electrophoresis result was used as length reference for the sequenced band and analyzed for methylation percentage and correlation with age estimation. Statistical test showed that there was a significant correlation between DNA methylation percentage with age estimation both in men (r Pearson 0.767) and women (r Pearson 0.878). Conclusion: Cpgplot emboss analysis for DNA methylation mean percentage in men tend to increase in accordance to age categories, whereas for women, the mean DNA methylation percentage in age categories was stable or constant.",

keywords = "Age estimation, Bite mark, DNA methylation",

author = "Ahmad Yudianto and {Mieke Sylvia Margaretta}, {A. R.} and Fery Setiawan and Ma{\textquoteright}Rifatul Ula",

note = "Funding Information: Sources of Funding: The author and the coauthors acknowledges to Rector of Universitas Airlangga that has given the funding to the program which called Hibah Penelitian Tesis Magister which number SK no 27/E1/KPT/2020. Publisher Copyright: {\textcopyright} 2021, Institute of Medico-Legal Publications. All rights reserved.",

year = "2021",

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doi = "10.37506/ijfmt.v15i1.13500",

language = "English",

volume = "15",

pages = "706--713",

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TY - JOUR

T1 - Human age estimation through DNA metilation analysis method from bite mark samples in forensic identification

AU - Yudianto, Ahmad

AU - Mieke Sylvia Margaretta, A. R.

AU - Setiawan, Fery

AU - Ula, Ma’Rifatul

N1 - Funding Information: Sources of Funding: The author and the coauthors acknowledges to Rector of Universitas Airlangga that has given the funding to the program which called Hibah Penelitian Tesis Magister which number SK no 27/E1/KPT/2020. Publisher Copyright: © 2021, Institute of Medico-Legal Publications. All rights reserved.

PY - 2021/1/1

Y1 - 2021/1/1

N2 - Background: Age estimation are often calculated using bone and teeth samples, but are limited to human skeleton findings. The aim of this research is to estimate human age through DNA methylation method from bite mark samples. Result: There were 40 bite mark samples obtained from healthy volunteers. DNA samples were isolated using DNAzol reagent and converted using DNA methylation kit. The isolated DNA were amplified and electrophoreses was conducted using agarose gel. Electrophoresis result was used as length reference for the sequenced band and analyzed for methylation percentage and correlation with age estimation. Statistical test showed that there was a significant correlation between DNA methylation percentage with age estimation both in men (r Pearson 0.767) and women (r Pearson 0.878). Conclusion: Cpgplot emboss analysis for DNA methylation mean percentage in men tend to increase in accordance to age categories, whereas for women, the mean DNA methylation percentage in age categories was stable or constant.

AB - Background: Age estimation are often calculated using bone and teeth samples, but are limited to human skeleton findings. The aim of this research is to estimate human age through DNA methylation method from bite mark samples. Result: There were 40 bite mark samples obtained from healthy volunteers. DNA samples were isolated using DNAzol reagent and converted using DNA methylation kit. The isolated DNA were amplified and electrophoreses was conducted using agarose gel. Electrophoresis result was used as length reference for the sequenced band and analyzed for methylation percentage and correlation with age estimation. Statistical test showed that there was a significant correlation between DNA methylation percentage with age estimation both in men (r Pearson 0.767) and women (r Pearson 0.878). Conclusion: Cpgplot emboss analysis for DNA methylation mean percentage in men tend to increase in accordance to age categories, whereas for women, the mean DNA methylation percentage in age categories was stable or constant.

KW - Age estimation

KW - Bite mark

KW - DNA methylation

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M3 - Article

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VL - 15

SP - 706

EP - 713

JO - Indian Journal of Forensic Medicine and Toxicology

JF - Indian Journal of Forensic Medicine and Toxicology

IS - 1

ER -

Human age estimation through DNA metilation analysis method from bite mark samples in forensic identification

Abstract

Keywords

UN SDGs

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