Heat stress has been found to cause adverse effects on small intestinal microstructure, but little is known about its impact on chicken's cecum. In this research, the histopathological alterations of broiler chicken's cecum following chronic heat stress were evaluated. 20 broiler chickens were randomly divided into control group and treatment group containing 10 replicates, respectively. Both groups were reared under standard conditions until 21 days of age. From day 22 to day 42, the control group was kept at 24-28°C as well as relative humidity of 40-55%, while the treatment group was exposed to high temperature of 36-40°C and relative humidity of 45-65% for eight hours per day. At the end of the period, proximal part of each chicken's cecum was collected and made into histopathological slides with Hematoxylin and Eosin staining. Villus height, villus width, crypt depth, villus surface area, and villus height to crypt depth ratio were examined from 10 villi per replicate. Results analysis revealed that chronic heat stress profoundly (P<0.05) reduced the crypt depth. Insignificant (P>0.05) changes of the villus despite the long-term heat exposure might imply that the damage is at its early phase. In conclusion, chronic heat stress can produce morphological alterations in the ceca of broiler chickens, though requiring longer duration due to cecum's durability.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalWorld's Veterinary Journal
Issue number3
Publication statusPublished - 1 Sept 2019


  • Broiler chicken
  • Cecum
  • Heat stress
  • Intestinal morphology


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