Histomorphometric Analysis of Anti-Aging Properties on Rat Skin

Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, Eka Pramyrtha Hestianah

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)

Abstract

We present a histomorphometric analysis to enable quantitative measurement of anti-aging activity of topical substances in rat skin. In this method, the measured parameters were closely related to changes that occur on the skin caused by exposure to UV light. We used this protocol to determine the anti-aging activities of an ethanolic extract of Curcuma heyneana rhizome and retinoic acid by a measurement of the changes in epidermal thickness, the number of sunburned cells, the number of fibroblasts, and the space between collagen fibers.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages313-321
Number of pages9
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2138
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Anti-aging assay
  • Curcuma heyneana rhizome
  • Histomorphometric analysis
  • Skin
  • UV rays

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