Histomorphometric Analysis of Anti-Aging Properties on Rat Skin

Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, Eka Pramyrtha Hestianah

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)


We present a histomorphometric analysis to enable quantitative measurement of anti-aging activity of topical substances in rat skin. In this method, the measured parameters were closely related to changes that occur on the skin caused by exposure to UV light. We used this protocol to determine the anti-aging activities of an ethanolic extract of Curcuma heyneana rhizome and retinoic acid by a measurement of the changes in epidermal thickness, the number of sunburned cells, the number of fibroblasts, and the space between collagen fibers.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Anti-aging assay
  • Curcuma heyneana rhizome
  • Histomorphometric analysis
  • Skin
  • UV rays


Dive into the research topics of 'Histomorphometric Analysis of Anti-Aging Properties on Rat Skin'. Together they form a unique fingerprint.

Cite this