Failure of hemostasis post-tooth extraction procedures can cause secondary bleeding and interfere wound healing process. Essential factors for the hemostasis process were platelet counts and fibrin density. Hibiscus rosa-sinensis Linn extract has been widely studied and proven increased the wound healing process. This study aimed to explore the effect of Hibiscus rosa-sinensis Linn in the post-tooth extraction during the hemostasis process by examining platelet counts and fibrin density. In vivo experimental study with five groups post-extraction socket of Rattus norvegicus. Two control groups without treatment and 3% CMC-Na gel-based group. The treatment groups received treatment with Hibiscus rosa-sinensis Linn leaf extract gel (HrsLE) with different concentrations, namely 10% HrsLE, 20% HrsLE, and 40% HrsLE. Blood clots from the extraction socket were evaluated for platelet counts and fibrin density using histopathological examination under a light microscope. Platelet counts were considered manually using Giemsa staining and fibrin density examination using Masson's trichrome staining. Data were analysed statistically using ANOVA for platelet counts and Kruskal Wallis for fibrin density, with a significance level of p<0.05 considered significant. The highest platelet counts and fibrin density were found in the 40% HrsLE group. All treatment groups were significantly higher for platelet and fibrin density than control (p<0.001 and p<0.0001). Among all, 40% HrsLE the highest count for platelet and fibrin density (p<0.0001). 40% HrsLE promote the hemostasis process post-tooth extraction by increase the platelet counts and fibrin density in socket.

Original languageEnglish
Pages (from-to)1086-1090
Number of pages5
JournalJournal of International Dental and Medical Research
Issue number3
Publication statusPublished - 2023


  • Affordable Medicines
  • Fibrin Density
  • Hemostasis Agent
  • Hibiscus rosa-sinensis Linn
  • Platelet Counts


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