TY - JOUR
T1 - Hibiscus Rosa-Sinensis Linn Leaf Extract Increased Platelet Counts and Fibrin Density
T2 - in Vivo Study
AU - Barus, Liska
AU - Arfianti, Novalia Syafira
AU - Surboyo, Meircurius Dwi Condro
AU - Harijadi, Achmad
AU - Danudiningrat, Coen Pramono
AU - Sumarta, Ni Putu Mira
N1 - Funding Information:
This work was supported by the Research Group Grant of Faculty of Dental Medicine Universitas Airlangga, fiscal year 2022.
Publisher Copyright:
© (2023), (University of Dicle). All Rights Reserved.
PY - 2023
Y1 - 2023
N2 - Failure of hemostasis post-tooth extraction procedures can cause secondary bleeding and interfere wound healing process. Essential factors for the hemostasis process were platelet counts and fibrin density. Hibiscus rosa-sinensis Linn extract has been widely studied and proven increased the wound healing process. This study aimed to explore the effect of Hibiscus rosa-sinensis Linn in the post-tooth extraction during the hemostasis process by examining platelet counts and fibrin density. In vivo experimental study with five groups post-extraction socket of Rattus norvegicus. Two control groups without treatment and 3% CMC-Na gel-based group. The treatment groups received treatment with Hibiscus rosa-sinensis Linn leaf extract gel (HrsLE) with different concentrations, namely 10% HrsLE, 20% HrsLE, and 40% HrsLE. Blood clots from the extraction socket were evaluated for platelet counts and fibrin density using histopathological examination under a light microscope. Platelet counts were considered manually using Giemsa staining and fibrin density examination using Masson's trichrome staining. Data were analysed statistically using ANOVA for platelet counts and Kruskal Wallis for fibrin density, with a significance level of p<0.05 considered significant. The highest platelet counts and fibrin density were found in the 40% HrsLE group. All treatment groups were significantly higher for platelet and fibrin density than control (p<0.001 and p<0.0001). Among all, 40% HrsLE the highest count for platelet and fibrin density (p<0.0001). 40% HrsLE promote the hemostasis process post-tooth extraction by increase the platelet counts and fibrin density in socket.
AB - Failure of hemostasis post-tooth extraction procedures can cause secondary bleeding and interfere wound healing process. Essential factors for the hemostasis process were platelet counts and fibrin density. Hibiscus rosa-sinensis Linn extract has been widely studied and proven increased the wound healing process. This study aimed to explore the effect of Hibiscus rosa-sinensis Linn in the post-tooth extraction during the hemostasis process by examining platelet counts and fibrin density. In vivo experimental study with five groups post-extraction socket of Rattus norvegicus. Two control groups without treatment and 3% CMC-Na gel-based group. The treatment groups received treatment with Hibiscus rosa-sinensis Linn leaf extract gel (HrsLE) with different concentrations, namely 10% HrsLE, 20% HrsLE, and 40% HrsLE. Blood clots from the extraction socket were evaluated for platelet counts and fibrin density using histopathological examination under a light microscope. Platelet counts were considered manually using Giemsa staining and fibrin density examination using Masson's trichrome staining. Data were analysed statistically using ANOVA for platelet counts and Kruskal Wallis for fibrin density, with a significance level of p<0.05 considered significant. The highest platelet counts and fibrin density were found in the 40% HrsLE group. All treatment groups were significantly higher for platelet and fibrin density than control (p<0.001 and p<0.0001). Among all, 40% HrsLE the highest count for platelet and fibrin density (p<0.0001). 40% HrsLE promote the hemostasis process post-tooth extraction by increase the platelet counts and fibrin density in socket.
KW - Affordable Medicines
KW - Fibrin Density
KW - Hemostasis Agent
KW - Hibiscus rosa-sinensis Linn
KW - Platelet Counts
UR - http://www.scopus.com/inward/record.url?scp=85173535566&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85173535566
SN - 1309-100X
VL - 16
SP - 1086
EP - 1090
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 3
ER -