Haplotype network analysis and phylogenetic tree construction of hepatitis c virus (Hcv) isolated from tuban, indonesia

Supiana Dian Nurtjahyani, Rasyadan Taufiq Probojati, Arif Nur Muhammad Ansori, Mochammad Amin, Retno Handajani

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


This study aimed to analyzed the haplotype network and constructed the phylogenetic tree topology of hepatitis C virus (HCV) in Tuban, Indonesia and those from other countries, to determine the visualize genealogical relationship and inference about gene connected. The HCV isolates were collected from blood transfusion center of Tuban, East Java, Indonesia in 2015 and as a comparison, also the sequences of HCV isolates were retrieved from the GenBank®, National Center of Biotechnology Information (NCBI), USA database. To constructed of distribution map was performed through median joining analysis using Haplotype Network v4.6, whereas to constructed and phylogenetic model analyses were constructed based on the NS5B and 5’UTR regions using MEGA X, maximum-likelihood method based on the Tamura-Nei model. In the HCV NS5B region haplotype network showed high haplotype diversity (Hd=1.00) in 22 haplotypes. Based on phylogenetic analysis 3 sample isolates Tuban (BDT-55-p23, BDT-79-p23 and BDT-112-p23) were identified one group and closely related to isolates from Indonesia. However, isolates Tuban also have the same sequence characters as the isolates from Thailand. It was also confirmed in haplotype network that the three isolates are connected to each other. The identification of HCV genotypes circulating in blood donors in the Tuban of East Java confirmed were closely related to HCV isolates from Indonesia and Thailand. Therefore, this result might contribute in a better medical management towards HCV.

Original languageEnglish
Pages (from-to)4231-4235
Number of pages5
JournalResearch Journal of Pharmacy and Technology
Issue number8
Publication statusPublished - Aug 2021


  • Haplotype Network Analysis
  • Hepatitis C virus
  • Phylogenetic Tree


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