Gynura procumbens Methanolic Extracts Suppresses Proliferation of Hepatocellular Carcinoma: In Vitro Assay

Sugiharto, Ummi Zubaidah, Dwi Winarni, Yosephine Sri Wulan Manuhara

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Nowadays, Hepatocellular Carcinoma (HCC) is regarded as one of the most frequent human neoplasm which causes the second and sixth most deaths in the world and Indonesia, respectively. HCC current medications are diverse with all their side effects. Thus, the discovery of low risk-novel therapeutic is important. Gynura procumbens is a perennial herb that has been suggested as anticancer therapeutic. In this current research, the toxicity effect of G. procumbens leaf methanolic extract (GPM) on HCC cell line (Huh7it) proliferation will be analyzed using MTT assay. To perform toxicity assay, GPM extract which contains 0.1% (v/v) dimethyl sulfoxide (DMSO) mixed with DMEM medium in various concentration. Huh7it cell line was seeded in DMEM medium at the density 2.3 x 104 cells/well then incubated overnight. Percentage (%) cell viability data from MTT assay were fitted to a logarithmic equation to determine IC50 value of GPM. The result showed that GPM possesses anti-proliferative activity on cell proliferation in dose-dependent manner but did not in time-dependent manner. The IC50 value of GPM was categorized as moderate where the IC50 value after 24 hours incubation was 63.83 |rg/mL while after 48 hours incubation was 125.84 |rg/mL. This study reveals that GPM potentially used as anticancer therapeutics in treating HCC.

Original languageEnglish
Title of host publication8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021
EditorsAnjar Tri Wibowo, M. Fariz Fadillah Mardianto, Riries Rulaningtyas, Satya Candra Wibawa Sakti, Muhammad Fauzul Imron, Rico Ramadhan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735442610
DOIs
Publication statusPublished - 25 Jan 2022
Event8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021 - Surabaya, Indonesia
Duration: 25 Aug 202126 Aug 2021

Publication series

NameAIP Conference Proceedings
Volume2554
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021
Country/TerritoryIndonesia
CitySurabaya
Period25/08/2126/08/21

Fingerprint

Dive into the research topics of 'Gynura procumbens Methanolic Extracts Suppresses Proliferation of Hepatocellular Carcinoma: In Vitro Assay'. Together they form a unique fingerprint.

Cite this