TY - JOUR
T1 - Gynura procumbens Adventitious Root Extract Altered Expression of Antioxidant Genes and Exert Hepatoprotective Effects Against Cadmium-Induced Oxidative Stress in Mice
AU - Sugiharto,
AU - Winarni, Dwi
AU - Wibowo, Anjar Tri
AU - Islamatasya, Ufairanisa
AU - Bhakti, Idqa Nurtri
AU - Nisa, Nabilatun
AU - Tan, Boon Chin
AU - Manuhara, Yosephine Sri Wulan
N1 - Funding Information:
We gratefully acknowledge to Universitas Airlangga for supporting via a Mandatory Research grant (No.1408/UN3/2019) and Ministry of Education and Culture (PDD grant No. 853/UN3.14/PT/2020).
Publisher Copyright:
© 2022, Bogor Agricultural University. All rights reserved.
PY - 2022/7
Y1 - 2022/7
N2 - Exposure to cadmium (Cd) could increase of reactive oxygen species (ROS) and changes in expression of antioxidant genes. Gynura procumbens is a medicinal plant that is rich in phenolic and flavonoid compounds. The aimed of study to evaluate the hepatoprotective effect of G. procumbens adventitious root (GAR) extract against Cd toxicity, especially expression rate of hepatic antioxidant genes. Twenty-five male mice were treated as follows: P1 (control), P2 (Cd100mg/L), P3 (GAR100mg/L + Cd100mg/L), P4 (GAR200mg/L + Cd100mg/L), and P5 (GAR300mg/L + Cd100mg/L). The samples (blood and liver) were collected for analysis of malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activities, and their relative gene expression were determined. The hematological assay showed Cd-treated administered with GAR extract increased the number of red blood cells (RBC), haemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), and mean corpuscular haemoglobin (MCH), but reduced the level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, the GAR extract decreased the MDA production, but increased the activities of SOD and CAT. These enzymatic activities were positively correlated with their respective gene transcripts. Our study revealed that GAR extract administration showed marked hepatoprotective effects against Cd-induced oxidative stress.
AB - Exposure to cadmium (Cd) could increase of reactive oxygen species (ROS) and changes in expression of antioxidant genes. Gynura procumbens is a medicinal plant that is rich in phenolic and flavonoid compounds. The aimed of study to evaluate the hepatoprotective effect of G. procumbens adventitious root (GAR) extract against Cd toxicity, especially expression rate of hepatic antioxidant genes. Twenty-five male mice were treated as follows: P1 (control), P2 (Cd100mg/L), P3 (GAR100mg/L + Cd100mg/L), P4 (GAR200mg/L + Cd100mg/L), and P5 (GAR300mg/L + Cd100mg/L). The samples (blood and liver) were collected for analysis of malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activities, and their relative gene expression were determined. The hematological assay showed Cd-treated administered with GAR extract increased the number of red blood cells (RBC), haemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), and mean corpuscular haemoglobin (MCH), but reduced the level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, the GAR extract decreased the MDA production, but increased the activities of SOD and CAT. These enzymatic activities were positively correlated with their respective gene transcripts. Our study revealed that GAR extract administration showed marked hepatoprotective effects against Cd-induced oxidative stress.
KW - Antioxidant
KW - Cadmium
KW - Gene expression
KW - Gynura procumbens
KW - Health care
KW - Hepatoprotective
UR - http://www.scopus.com/inward/record.url?scp=85130831112&partnerID=8YFLogxK
U2 - 10.4308/hjb.29.4.479-486
DO - 10.4308/hjb.29.4.479-486
M3 - Article
AN - SCOPUS:85130831112
SN - 1978-3019
VL - 29
SP - 479
EP - 486
JO - HAYATI Journal of Biosciences
JF - HAYATI Journal of Biosciences
IS - 4
ER -