Grape seed extract increase osteoblast number in the post-extraction socket healing in rats

Olivia Jennifer Gunardi, A. Agustina Putri Kintan, R. Soesanto, Ni Putu Mira Sumarta

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Delayed wound healing after tooth extraction causes infections and pains. According to previous studies, grape seed extract accelerates surgical wounds healing process due to its anti-oxidant properties, which triggers the release of vascular endothelial growth factor along with the promotion of fibroblasts to produce more collagen fibres. Furthermore, its anti-inflammatory and anti-microbial properties are effective in wound healing. This study aimed to examine the increased osteoblast as a bone healing indicator in post-extraction socket in wistar rat after the application of grape seed. An experimental study was conducted on four groups of wistar rat, each of which consisted of 6 rats whose lower left first incisor were extracted. The first and second groups which served as the control groups (C) without the application of grape seed extract after tooth extraction was evaluated for 7 (C1) and 14 days (C2). The third and fourth group were the experimental groups (E) with 0.1 ml of 2% grape seed extract administered and evaluated for 7 (E1) and 14 days (E2) respectively. Osteoblast numbers were evaluated using histological examinations with hematoxylin-eosin stains which were observed under light microscope. Furthermore, statistical analysis were carried out using Independent T-test with SPSS 16th version, with a significance level of p<0.005. The results showed that there were significant difference between the control and experiment groups with p = 0.000, with a comparative test between the experimental E1 and E2 groups showing a significant difference of p = 0.002. Grape seed extract tends to increase osteoblast proliferation in post extraction socket.

Original languageEnglish
Pages (from-to)4877-4881
Number of pages5
JournalBiochemical and Cellular Archives
Publication statusPublished - 1 Dec 2019


  • Grape seed extract
  • Osteoblast induction factor
  • Tooth extractions
  • Wound healing


Dive into the research topics of 'Grape seed extract increase osteoblast number in the post-extraction socket healing in rats'. Together they form a unique fingerprint.

Cite this