TY - JOUR
T1 - Gingiva Mesenchymal Stem Cells Normoxic or Hypoxic Preconditioned Application Under Orthodontic Mechanical Force on Osterix, Osteopontin, and ALP Expression
AU - Nugraha, Alexander Patera
AU - Narmada, Ida Bagus
AU - Winoto, Ervina Restiwulan
AU - Ardani, I. Gusti Aju Wahju
AU - Triwardhani, Ari
AU - Alida, Alida
AU - Pramusita, Adya
AU - Nur, Reyhan Mahendra
AU - Indrastie, Nuraini
AU - Nam, Hui Yin
AU - Ihsan, Igo Syaiful
AU - Riawan, Wibi
AU - Rantam, Fedik Abdul
AU - Nugraha, Albertus Putera
AU - Noor, Tengku Natasha Eleena Binti Tengku Ahmad
N1 - Publisher Copyright:
© 2023. The Author(s).
PY - 2024/5/28
Y1 - 2024/5/28
N2 - Objectives The aim of this article was to investigate Osterix, ALP, and osteopontin expression in the compression and tension sides of alveolar bone after the application of normoxic/hypoxic-preconditioned GMSCs in rabbits (Oryctolagus cuniculus) induced with OMF. Materials and Methods Forty-eight healthy, young male rabbits were divided into four groups: [-] OMF; [+] OMF; OMF with GMSCs normoxic-preconditioned; and OMF and GMSCs hypoxic-preconditioned. The central incisor and left mandibular molar in the experimental animals were moved, the mandibular first molar was moved mesially using nickel titanium (NiTi) and stainless steel ligature wire connected to a 50 g/mm 2 light force closed coil spring. Allogeneic application of normoxic or hypoxic-preconditioned GMSCs was used in as many as 10 6 cells in a 20 μL phosphate buffered saline single dose and injected into experimental animals' gingiva after 1 day of OTM. On days 7, 14, and 28, all experimental animals were euthanized. Osterix, ALP, and osteopontin expressions were examined by immunohistochemistry. Results Osterix, ALP, and osteopontin expressions were significantly different after allogeneic application of hypoxic-preconditioned GMSCs than normoxic-preconditioned GMSCs in the tension and compression of the alveolar bone side during OMF (p < 0.05). Conclusion Osterix, ALP, and osteopontin expressions were significantly more enhanced post-transplantation of GMSCs with hypoxic-preconditioning than after transplantation of normoxic-preconditioned GMSCs in rabbits (O. cuniculus) induced with OMF.
AB - Objectives The aim of this article was to investigate Osterix, ALP, and osteopontin expression in the compression and tension sides of alveolar bone after the application of normoxic/hypoxic-preconditioned GMSCs in rabbits (Oryctolagus cuniculus) induced with OMF. Materials and Methods Forty-eight healthy, young male rabbits were divided into four groups: [-] OMF; [+] OMF; OMF with GMSCs normoxic-preconditioned; and OMF and GMSCs hypoxic-preconditioned. The central incisor and left mandibular molar in the experimental animals were moved, the mandibular first molar was moved mesially using nickel titanium (NiTi) and stainless steel ligature wire connected to a 50 g/mm 2 light force closed coil spring. Allogeneic application of normoxic or hypoxic-preconditioned GMSCs was used in as many as 10 6 cells in a 20 μL phosphate buffered saline single dose and injected into experimental animals' gingiva after 1 day of OTM. On days 7, 14, and 28, all experimental animals were euthanized. Osterix, ALP, and osteopontin expressions were examined by immunohistochemistry. Results Osterix, ALP, and osteopontin expressions were significantly different after allogeneic application of hypoxic-preconditioned GMSCs than normoxic-preconditioned GMSCs in the tension and compression of the alveolar bone side during OMF (p < 0.05). Conclusion Osterix, ALP, and osteopontin expressions were significantly more enhanced post-transplantation of GMSCs with hypoxic-preconditioning than after transplantation of normoxic-preconditioned GMSCs in rabbits (O. cuniculus) induced with OMF.
KW - alveolar bone
KW - hypoxia
KW - medicine
KW - orthodontic tooth movement
KW - stem cells
UR - http://www.scopus.com/inward/record.url?scp=85179400220&partnerID=8YFLogxK
U2 - 10.1055/s-0043-1772699
DO - 10.1055/s-0043-1772699
M3 - Article
AN - SCOPUS:85179400220
SN - 1305-7456
VL - 18
SP - 501
EP - 509
JO - European Journal of Dentistry
JF - European Journal of Dentistry
IS - 2
ER -