Introduction: One of the main factors supporting obesity is the disruption in the performance of the circadian rhythm which results in a decrease in the quality and quantity of sleep, which triggers stress which is regulated by the hormone serotonin in the body. This increase in serotonin is related to factors that trigger the risk of obesity, besides that melatonin and leptin in the body can decrease, thereby increasing ghrelin which stimulates appetite. The aim of this study was to prove that ghrelin and serotonin can be used as indicators of obesity in Wistar rats as experimental animals. Materials and Methods: This study involved 3 groups with each group consisting of 6 samples. Group 1 was the normal group (12 hours light, 12 hours dark), group 2 was the dark group (24 hours dark) and group 3 was the light group (24 hours light. Each group was treated with circadian and modified feed until obesity was found and then the blood measured using the Rat ELISA Kit. Results: The results of the correlation test of body weight of Wistar rats with ghrelin and serotonin show that there is a strong relationship between body weight and ghrelin with p-value = 0.006 (p< 0.05), r= 0.609. The correlation between body weight and serotonin was moderate with p-value = 0.023 (p< 0.05), r= 0.517. Conclusion: Ghrelin and Serotonin can be used as indicators of obesity in Wistar rats.

Original languageEnglish
Pages (from-to)38-42
Number of pages5
JournalMalaysian Journal of Medicine and Health Sciences
Publication statusPublished - Feb 2023


  • Circadian rhythm
  • Ghrelin
  • Obesity
  • Serotonin


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