Genotyping of HLA-DRB1 by PCR-SSP in Javanese patients with Recurrent Aphthous Stomatitis

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Recurrent Aphthous Stomatitis (RAS) is the most common inflammatory ulcerative condition of the oral mucosa. Association of genetic factor and recurrent aphthous stomatitis has been implicated by numerous studies and this can be determined by HLA subtypes. The present study was conducted to determine the possible association of Class II alleles with recurrent aphthous stomatitis in Javanese patients. The study included 63 subjects i.e. 42 subjects were suffering from recurrent aphthous stomatitis and 21 healthy subjects were included as control. Polymerase chain reaction-specific sequence primers (PCR-SSP-Morgan TM) assay was conducted to assess HLA-typing. Genotyping of subjects for HLA class II revealed that HLA-DRB1∗15:01:01:01; DRB1∗15:01:01:01-6; HLA-DRB1∗3:01:01:01 and DRB1∗07:01:01:01 genes were predominant with high frequency alleles in RAS patients. The present study showed a significant association of DRB1∗16:01:01:05/DR16 alleles with RAS as compared with control subjects. In conclusion, this study demonstrated that gene HLA-DRB1 has predominant allele and has important role in etiology of the disease especially in the Javanese people.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalBioscience Research
Issue number1-2
Publication statusPublished - 2015


  • Aphthous stomatitis
  • HLA alleles
  • RAS


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