Genetic Variation using the 21 Str Codis Loci for Forensic Identification Examinations among Siblings of Madurese Living in Surabaya

Indonesia consists of various ethnic groups. However, well-established research on the genetic variation of each ethnic group in Indonesia has not yet been present. Madurese is one of the Indonesian ethnic groups inhabiting Madura island and living in remote areas throughout Indonesia. Surabaya- Madura's mobilization is tremendous as the closest city to Madura Island, connected by a bridge. The Madurese has a culture of didi and same-ethnic marriage patterns. Therefore, research was conducted on 21 STR panel loci to analyze Madurese living in Surabaya to increase genetic data libraries and genetic variation of Indonesian populations based on siblings. PrepFiler kit to extract DNA from blood and GlobalFiler kit to amplify 21 loci were used. An ABI PRISM 3500 genetic analyzer was used to detect PCR products. Data were processed and analyzed with EasyDNA software and FORSTAT. Allele frequency data for 21 autosomal loci in Madurese were obtained. The expected Heterozygosity (He) was found with the highest obtained at locus D19S433 (0.911), while the lowest was at locus D22S1045 (0.607). Power of Discrimination (PD) values ranged from the highest at 2 loci, namely D5S818, and D13S317 (0.968), while the lowest at locus D2S1338 (0.857). Polymorphism Information Content (PIC) value is highest at locus D22S1045 (0.765); all loci have PIC values?> 0.5. The Sibship Index (SI) value of the Madurese in Surabaya is 0.88 – 1.20. The 21 STR loci can be employed for population genetic variation studies and sibling forensic identification.