Genetic similarity analysis of Vanda tricolor Lindley var. Suavis orchids using OPU 03 and OPU 16 RAPD marker

The purpose of this research is to understand the genetic similarity level of Vanda tricolor Lindley var. Suavis orchid plants that were regenerated from seeds of the same fruit/pod produced by self-pollination. The seeds were sown on New Phalaenopsis (NP) in vitro culture medium supplemented with 15 % coconut water (CW). Genetic similarity analysis was performed by PCR-based Random Amplification of Polymorphic DNA (PCR-RAPD) technique. The analysis was carried out at 16 months old regenerated plants after sowing (mas). Seven DNA samples were isolated using a modified Castillo method. It was then used to amplify (PCR) OPU 3 and OPU 16 primers. The electropherogram of PCR result was then converted to binary data and analysed using NTSYSpc software version 2.02. The genetic analysis result showed that the genetic similarity of 7 tested plants ranged between 0.4-0.8.