Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker

Fitria Permatasari; Ixora S. Mercuriani; Evy Yulianti

doi:10.1063/1.5062755

Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker

Fitria Permatasari, Ixora S. Mercuriani, Evy Yulianti

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

Rhynchostylis retusa (L.) Blume is a monopodial epiphytic orchid species with attractive flowers arranged in racemose inflorescence. Mass propagation of wild type orchids were generally done by regenerated seeds from the same fruit. The study aimed to know the genetic similarity level of R. retusa plants which are regenerated from the same fruit seeds produced by self-pollination. The seeds were sown on New Phaleonopsis (NP) in vitro culture medium supplemented by 1.5% coconut water (CO). Genetic similarity levels were analyzed using PCR based Random Amplification of DNA Polimorfism (PCR-RAPD) with OPA 15 and OPA 03 primers. The analysis was performed 19 months after sowing the seed (m.a.s). DNA were isolated from 10 plants using a modified Castillo method. The electroforegram of the PCR result was converted to binary data and analyzed using NTSYSpc software version 2.02. The DNA analysis shows that all plants which were tested have a high similarity of DNA character.

Original language	English
Title of host publication	8th Annual Basic Science International Conference
Subtitle of host publication	Coverage of Basic Sciences toward the World's Sustainability Challenges
Editors	Corina Karim, Rodliyati Azrianingsih, Mauludi Ariesto Pamungkas, Yoga Dwi Jatmiko, Anna Safitri
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735417397
DOIs	https://doi.org/10.1063/1.5062755
Publication status	Published - 17 Oct 2018
Externally published	Yes
Event	8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018 - Malang, East Java, Indonesia Duration: 6 Mar 2018 → 7 Mar 2018

Publication series

Name	AIP Conference Proceedings
Volume	2021
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018
Country/Territory	Indonesia
City	Malang, East Java
Period	6/03/18 → 7/03/18

Keywords

RAPD
Rhynchostylis retusa
genetic similarity
in vitro culture

Access to Document

10.1063/1.5062755

Cite this

Permatasari, F., Mercuriani, I. S., & Yulianti, E. (2018). Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker. In C. Karim, R. Azrianingsih, M. A. Pamungkas, Y. D. Jatmiko, & A. Safitri (Eds.), 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challenges Article 050005 (AIP Conference Proceedings; Vol. 2021). American Institute of Physics Inc.. https://doi.org/10.1063/1.5062755

Permatasari, Fitria ; Mercuriani, Ixora S. ; Yulianti, Evy. / Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker. 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challenges. editor / Corina Karim ; Rodliyati Azrianingsih ; Mauludi Ariesto Pamungkas ; Yoga Dwi Jatmiko ; Anna Safitri. American Institute of Physics Inc., 2018. (AIP Conference Proceedings).

@inproceedings{5a8f3647e9c94edd9fb7c03ae64067d8,

title = "Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker",

abstract = "Rhynchostylis retusa (L.) Blume is a monopodial epiphytic orchid species with attractive flowers arranged in racemose inflorescence. Mass propagation of wild type orchids were generally done by regenerated seeds from the same fruit. The study aimed to know the genetic similarity level of R. retusa plants which are regenerated from the same fruit seeds produced by self-pollination. The seeds were sown on New Phaleonopsis (NP) in vitro culture medium supplemented by 1.5% coconut water (CO). Genetic similarity levels were analyzed using PCR based Random Amplification of DNA Polimorfism (PCR-RAPD) with OPA 15 and OPA 03 primers. The analysis was performed 19 months after sowing the seed (m.a.s). DNA were isolated from 10 plants using a modified Castillo method. The electroforegram of the PCR result was converted to binary data and analyzed using NTSYSpc software version 2.02. The DNA analysis shows that all plants which were tested have a high similarity of DNA character.",

keywords = "RAPD, Rhynchostylis retusa, genetic similarity, in vitro culture",

author = "Fitria Permatasari and Mercuriani, {Ixora S.} and Evy Yulianti",

note = "Funding Information: This research was supported by Universitas Negeri Yogyakarta. We like to show our gratitude to Mrs. Ratnawati, Prof. Djukri, Mrs. Lili for sharing their pearls of wisdom with us during the course of this research, and we thank Tonny Haryo also Ulfia Nurul for providing assistances on this research so we could presented well. Publisher Copyright: {\textcopyright} 2018 Author(s).; 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018 ; Conference date: 06-03-2018 Through 07-03-2018",

year = "2018",

month = oct,

day = "17",

doi = "10.1063/1.5062755",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Corina Karim and Rodliyati Azrianingsih and Pamungkas, {Mauludi Ariesto} and Jatmiko, {Yoga Dwi} and Anna Safitri",

booktitle = "8th Annual Basic Science International Conference",

}

Permatasari, F, Mercuriani, IS & Yulianti, E 2018, Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker. in C Karim, R Azrianingsih, MA Pamungkas, YD Jatmiko & A Safitri (eds), 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challenges., 050005, AIP Conference Proceedings, vol. 2021, American Institute of Physics Inc., 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018, Malang, East Java, Indonesia, 6/03/18. https://doi.org/10.1063/1.5062755

Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker. / Permatasari, Fitria; Mercuriani, Ixora S.; Yulianti, Evy.
8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challenges. ed. / Corina Karim; Rodliyati Azrianingsih; Mauludi Ariesto Pamungkas; Yoga Dwi Jatmiko; Anna Safitri. American Institute of Physics Inc., 2018. 050005 (AIP Conference Proceedings; Vol. 2021).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker

AU - Permatasari, Fitria

AU - Mercuriani, Ixora S.

AU - Yulianti, Evy

N1 - Funding Information: This research was supported by Universitas Negeri Yogyakarta. We like to show our gratitude to Mrs. Ratnawati, Prof. Djukri, Mrs. Lili for sharing their pearls of wisdom with us during the course of this research, and we thank Tonny Haryo also Ulfia Nurul for providing assistances on this research so we could presented well. Publisher Copyright: © 2018 Author(s).

PY - 2018/10/17

Y1 - 2018/10/17

N2 - Rhynchostylis retusa (L.) Blume is a monopodial epiphytic orchid species with attractive flowers arranged in racemose inflorescence. Mass propagation of wild type orchids were generally done by regenerated seeds from the same fruit. The study aimed to know the genetic similarity level of R. retusa plants which are regenerated from the same fruit seeds produced by self-pollination. The seeds were sown on New Phaleonopsis (NP) in vitro culture medium supplemented by 1.5% coconut water (CO). Genetic similarity levels were analyzed using PCR based Random Amplification of DNA Polimorfism (PCR-RAPD) with OPA 15 and OPA 03 primers. The analysis was performed 19 months after sowing the seed (m.a.s). DNA were isolated from 10 plants using a modified Castillo method. The electroforegram of the PCR result was converted to binary data and analyzed using NTSYSpc software version 2.02. The DNA analysis shows that all plants which were tested have a high similarity of DNA character.

AB - Rhynchostylis retusa (L.) Blume is a monopodial epiphytic orchid species with attractive flowers arranged in racemose inflorescence. Mass propagation of wild type orchids were generally done by regenerated seeds from the same fruit. The study aimed to know the genetic similarity level of R. retusa plants which are regenerated from the same fruit seeds produced by self-pollination. The seeds were sown on New Phaleonopsis (NP) in vitro culture medium supplemented by 1.5% coconut water (CO). Genetic similarity levels were analyzed using PCR based Random Amplification of DNA Polimorfism (PCR-RAPD) with OPA 15 and OPA 03 primers. The analysis was performed 19 months after sowing the seed (m.a.s). DNA were isolated from 10 plants using a modified Castillo method. The electroforegram of the PCR result was converted to binary data and analyzed using NTSYSpc software version 2.02. The DNA analysis shows that all plants which were tested have a high similarity of DNA character.

KW - RAPD

KW - Rhynchostylis retusa

KW - genetic similarity

KW - in vitro culture

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U2 - 10.1063/1.5062755

DO - 10.1063/1.5062755

M3 - Conference contribution

AN - SCOPUS:85056199027

T3 - AIP Conference Proceedings

BT - 8th Annual Basic Science International Conference

A2 - Karim, Corina

A2 - Azrianingsih, Rodliyati

A2 - Pamungkas, Mauludi Ariesto

A2 - Jatmiko, Yoga Dwi

A2 - Safitri, Anna

PB - American Institute of Physics Inc.

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Permatasari F, Mercuriani IS, Yulianti E. Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker. In Karim C, Azrianingsih R, Pamungkas MA, Jatmiko YD, Safitri A, editors, 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challenges. American Institute of Physics Inc. 2018. 050005. (AIP Conference Proceedings). doi: 10.1063/1.5062755

Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker

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