Genetic polymorphism of pit-1|hinfi gene in grati-Ongole grade cattle at Indonesian beef cattle research station

H. Hartati; S. Anwar; B. D.P. Soewandi

doi:10.14710/jitaa.43.4.315-322

Genetic polymorphism of pit-1|hinfi gene in grati-Ongole grade cattle at Indonesian beef cattle research station

H. Hartati, S. Anwar, B. D.P. Soewandi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The pituitary transcription factor (Pit-1) gene is one of the considered genes that responsible to growth in cattle. A specific Pit-1|HinfI gene polymorphism located within exon 6 (g.1256G>A) has been shown to be associated with growth and milk traits in cattle. This study aimed to identify the Pit-1|Hinf1 gene polymorphism in Grati-Ongole Grade cattle (Grati-OG cattle). A total of 107 genomic DNA of Grati-OG cattle were used in this study. The detection of polymorphism was performed by PCR-RFLP method. Only AB and BB genotypes were succesfully identified with the frequency of 0.009 and 0.991, respectively. Whereas, the frequency of A and B alleles were 0.005 and 0.995, respectively. The observed genotype frequencies in this population do not deviated from HWE. The value of Ho, He and PIC were 0.009, 0.009 and 0.009, respectively. In conclusion, the Pit-1|HinfI gene polymorphism in Grati-OG cattle is monomorphic and hence it should not be used in further association studies.

Original language	English
Pages (from-to)	315-322
Number of pages	8
Journal	Journal of the Indonesian Tropical Animal Agriculture
Volume	43
Issue number	4
DOIs	https://doi.org/10.14710/jitaa.43.4.315-322
Publication status	Published - Dec 2018
Externally published	Yes

Keywords

Bos indicus
Gene polymorphism
Ongole Grade cattle
PCR-RFLP
Pit-1 gene

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10.14710/jitaa.43.4.315-322

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@article{952557c6221f4a578e57cf47583e6cf6,

title = "Genetic polymorphism of pit-1|hinfi gene in grati-Ongole grade cattle at Indonesian beef cattle research station",

abstract = "The pituitary transcription factor (Pit-1) gene is one of the considered genes that responsible to growth in cattle. A specific Pit-1|HinfI gene polymorphism located within exon 6 (g.1256G>A) has been shown to be associated with growth and milk traits in cattle. This study aimed to identify the Pit-1|Hinf1 gene polymorphism in Grati-Ongole Grade cattle (Grati-OG cattle). A total of 107 genomic DNA of Grati-OG cattle were used in this study. The detection of polymorphism was performed by PCR-RFLP method. Only AB and BB genotypes were succesfully identified with the frequency of 0.009 and 0.991, respectively. Whereas, the frequency of A and B alleles were 0.005 and 0.995, respectively. The observed genotype frequencies in this population do not deviated from HWE. The value of Ho, He and PIC were 0.009, 0.009 and 0.009, respectively. In conclusion, the Pit-1|HinfI gene polymorphism in Grati-OG cattle is monomorphic and hence it should not be used in further association studies.",

keywords = "Bos indicus, Gene polymorphism, Ongole Grade cattle, PCR-RFLP, Pit-1 gene",

author = "H. Hartati and S. Anwar and Soewandi, {B. D.P.}",

note = "Funding Information: This research was funded by Kerjasama Penelitian, Pengkajian dan Pengembangan Pertanian Strategis (KP4S) Research Program (Grant no. 90.22/HM.230/H.1/05/2017.K) from Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture of Indonesia. The authors are grateful to Lusiana, Viana Rahmawati and Septiana Tri Nugraheni for laboratory assistance and to all people who involved in blood sampling assistance. Publisher Copyright: {\textcopyright} 2018 Diponegoro University. All Rights Reserved.",

year = "2018",

month = dec,

doi = "10.14710/jitaa.43.4.315-322",

language = "English",

volume = "43",

pages = "315--322",

journal = "Journal of the Indonesian Tropical Animal Agriculture",

issn = "2087-8273",

publisher = "Diponegoro University",

number = "4",

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TY - JOUR

T1 - Genetic polymorphism of pit-1|hinfi gene in grati-Ongole grade cattle at Indonesian beef cattle research station

AU - Hartati, H.

AU - Anwar, S.

AU - Soewandi, B. D.P.

N1 - Funding Information: This research was funded by Kerjasama Penelitian, Pengkajian dan Pengembangan Pertanian Strategis (KP4S) Research Program (Grant no. 90.22/HM.230/H.1/05/2017.K) from Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture of Indonesia. The authors are grateful to Lusiana, Viana Rahmawati and Septiana Tri Nugraheni for laboratory assistance and to all people who involved in blood sampling assistance. Publisher Copyright: © 2018 Diponegoro University. All Rights Reserved.

PY - 2018/12

Y1 - 2018/12

N2 - The pituitary transcription factor (Pit-1) gene is one of the considered genes that responsible to growth in cattle. A specific Pit-1|HinfI gene polymorphism located within exon 6 (g.1256G>A) has been shown to be associated with growth and milk traits in cattle. This study aimed to identify the Pit-1|Hinf1 gene polymorphism in Grati-Ongole Grade cattle (Grati-OG cattle). A total of 107 genomic DNA of Grati-OG cattle were used in this study. The detection of polymorphism was performed by PCR-RFLP method. Only AB and BB genotypes were succesfully identified with the frequency of 0.009 and 0.991, respectively. Whereas, the frequency of A and B alleles were 0.005 and 0.995, respectively. The observed genotype frequencies in this population do not deviated from HWE. The value of Ho, He and PIC were 0.009, 0.009 and 0.009, respectively. In conclusion, the Pit-1|HinfI gene polymorphism in Grati-OG cattle is monomorphic and hence it should not be used in further association studies.

AB - The pituitary transcription factor (Pit-1) gene is one of the considered genes that responsible to growth in cattle. A specific Pit-1|HinfI gene polymorphism located within exon 6 (g.1256G>A) has been shown to be associated with growth and milk traits in cattle. This study aimed to identify the Pit-1|Hinf1 gene polymorphism in Grati-Ongole Grade cattle (Grati-OG cattle). A total of 107 genomic DNA of Grati-OG cattle were used in this study. The detection of polymorphism was performed by PCR-RFLP method. Only AB and BB genotypes were succesfully identified with the frequency of 0.009 and 0.991, respectively. Whereas, the frequency of A and B alleles were 0.005 and 0.995, respectively. The observed genotype frequencies in this population do not deviated from HWE. The value of Ho, He and PIC were 0.009, 0.009 and 0.009, respectively. In conclusion, the Pit-1|HinfI gene polymorphism in Grati-OG cattle is monomorphic and hence it should not be used in further association studies.

KW - Bos indicus

KW - Gene polymorphism

KW - Ongole Grade cattle

KW - PCR-RFLP

KW - Pit-1 gene

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AN - SCOPUS:85058672303

SN - 2087-8273

VL - 43

SP - 315

EP - 322

JO - Journal of the Indonesian Tropical Animal Agriculture

JF - Journal of the Indonesian Tropical Animal Agriculture

IS - 4

ER -

Genetic polymorphism of pit-1|hinfi gene in grati-Ongole grade cattle at Indonesian beef cattle research station

Abstract

Keywords

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