TY - JOUR
T1 - Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences
AU - Amrullah, Muhammad Fajar
AU - Utomo, Budi
AU - Utama, Suzanita
AU - Suprayogi, Tri Wahyu
AU - Lestari, Tita Damayanti
AU - Restiadi, Tjuk Imam
AU - Belgania, Ristaqul Husna
N1 - Publisher Copyright:
© 2023, Airlangga University. All rights reserved.
PY - 2023/4
Y1 - 2023/4
N2 - The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds.
AB - The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds.
KW - SNPs
KW - genetic diversity
KW - goat
KW - leptin gene
KW - phylogenetic tree
UR - http://www.scopus.com/inward/record.url?scp=85170530715&partnerID=8YFLogxK
U2 - 10.20473/jmv.vol6.iss1.2023.125-131
DO - 10.20473/jmv.vol6.iss1.2023.125-131
M3 - Article
AN - SCOPUS:85170530715
SN - 2615-7497
VL - 6
SP - 125
EP - 131
JO - Jurnal Medik Veteriner
JF - Jurnal Medik Veteriner
IS - 1
ER -