GCMS analysis of bioactive compounds in n-hexane, ethyl acetate, and methanol extract of Piper betle L. var. nigra

Black betel (Piper betle L. var nigra) is one of endemic Piperaceae species in Indonesia. So far, few information was available about its bioactive compounds content. Thus, this study was designed to identify bioactive compounds contained in n-hexane, ethyl acetate, and methanol extract of P. betle L. var. Nigra leaves. Symplicia of black betel was extracted from the leaves using three types of solvent, before being analyzed of its compound types and amounts using Gas Chromatography Mass Spectra (GCMS). Result showed that three main components found in n-hexane extract were 1-pentene (22.57%), 3-butenoic acid (18.29%), and furan (16.83%). Three main components of ethyl acetate extract were acetic acid (58.35%), methylamine (21.5%), and acetic acid (15.29%). In the other hand, methanol extract contained three main components of methylamine (85.08%), acetic acid (4.78%), and pyridine (0.21%).