TY - JOUR
T1 - Freeze-dried bovine and human amnion membrane properties
AU - Wijaksana, I. Komang Evan
AU - Ulfah, Noer
AU - Setiawatie, Ernie Maduratna
N1 - Publisher Copyright:
© 2020 Connect Journal.
PY - 2020
Y1 - 2020
N2 - The development of cell-based tissue engineering and cell transfer technology enables the incorporation of stem cells in scaffold that can help achieve periodontal tissue regeneration. Bovine amnion membrane (BAM) is an alternative to the human placental amnion membrane as a scaffold for adipose tissue stem cells. However, there has not been much research on the physical, chemical and mechanical properties of the Freeze-Dried Human Amnion Membrane (FDHAM) and Freeze-Dried Bovine Amnion Membrane (FDBAM). The purpose of this study is to analyze the properties of FDHAM and FDBAM.Experimental laboratory study to examine the physical properties of membrane thickness (by screw micrometer) and membrane porosity (SEM observation), chemical properties through elemental content (EDX observation) and functional group spectrophotometry (FTIR observation), mechanical properties through swelling ratio, water content percentage and degradation rate. FDBAM was thicker than FDHAM (p<0.05), but did not have differences in porosity size, elemental content, functional group spectrophotometry, swelling ratio, percentage of water content and degradation rate (P>0.05). FDBAM has no differences in physical, chemical and mechanical properties as FDHAM.
AB - The development of cell-based tissue engineering and cell transfer technology enables the incorporation of stem cells in scaffold that can help achieve periodontal tissue regeneration. Bovine amnion membrane (BAM) is an alternative to the human placental amnion membrane as a scaffold for adipose tissue stem cells. However, there has not been much research on the physical, chemical and mechanical properties of the Freeze-Dried Human Amnion Membrane (FDHAM) and Freeze-Dried Bovine Amnion Membrane (FDBAM). The purpose of this study is to analyze the properties of FDHAM and FDBAM.Experimental laboratory study to examine the physical properties of membrane thickness (by screw micrometer) and membrane porosity (SEM observation), chemical properties through elemental content (EDX observation) and functional group spectrophotometry (FTIR observation), mechanical properties through swelling ratio, water content percentage and degradation rate. FDBAM was thicker than FDHAM (p<0.05), but did not have differences in porosity size, elemental content, functional group spectrophotometry, swelling ratio, percentage of water content and degradation rate (P>0.05). FDBAM has no differences in physical, chemical and mechanical properties as FDHAM.
KW - Amnion
KW - Characteristic
KW - FDBAM
KW - FDHAM
KW - Medicine
UR - http://www.scopus.com/inward/record.url?scp=85091374311&partnerID=8YFLogxK
U2 - 10.35124/bca.2020.20.S1.2883
DO - 10.35124/bca.2020.20.S1.2883
M3 - Article
AN - SCOPUS:85091374311
SN - 0972-5075
VL - 20
SP - 2883
EP - 2889
JO - Biochemical and Cellular Archives
JF - Biochemical and Cellular Archives
ER -