Formation of woven bone in orthodontic tooth movement tension areas after giving Mangostin by expression of Runx-2 and IL-10

Background: Alveolar bone remodeling is very helpful in orthodontic treatment to prevent relapse after treatment is completed with new bone formation. Using natural materials that have a mechanism of action on the periodontal ligament and alveolar bone as a relapse prevention triggers osteogenesis with the formation of new bone in the tension area so that orthodontic treatment can be achieved maximally. This study aims to determine the formation of woven bone in the area attraction of orthodontic tooth movement after administration of Mangostin Methods: A total of 30 male Wistar rats were divided into 3 groups, namely a negative control group (K1) without treatment, K2 and K3 a positive control group K(+), K2 a positive control group K(+), which was given a mechanical stressor without Mangostin administration and observed for 7 days, K3 positive control group K(+) which was given a mechanical stressor without Mangostin administration and observed for 14 days, K4 and K5 treatment group (P), K4 treatment group (P) where the group was given a mechanical stressor and Mangostin which was observed for 7 days, K5 the treatment group (P) where the group was given a mechanical stressor and Mangostin which was observed for 14 days. Dara was analyzed using SPSS version 23 for Windows. Results: It was seen that the administration of Mangostin was very effective in increasing the expression of Runx-2, IL-10 and the formation of woven bone in the area of higher tension than without the administration of Mangostin significantly (p<0.05). There was a significant increase (p<0.05) of Runx-2 and IL-10 in the treated group compared to the control group on day 7 and day 14, respectively. Conclusion: The administration of Mangostin effectively prevented orthodontic relapse by increasing the expression of Runx-2 and Il-10 and accelerating the formation of woven bone in the tension area of orthodontic movement.