Folliculogenesis effect of Allium sativum, curcuma mangga and acorus calamus extracts on rats (Rattus norvegicus)

This study aims at determining the effect of the combination of Allium sativum, Curcuma mangga and Acorus calamus on ovarian folliculogenesis of rats (Rattus novergicus). This study used a completely randomized design (CRD) consisting of nine treatments and four replications. Treatment consisted of K-(negative control), K+ (clomiphene citrate dose of 0.9 mg/kg BW), P₁, P₂, P₃ (composition 1, dose of 50, 75 and 100 mg/kg BW), P₄, P₅, P₆ (composition 2, dose of 50, 75 and 100 mg/kg BW) and P₇ (Subur kandungan herb™, dose of 75 mg/kg BW). The combination of A. sativum, C. mangga and A. calamus affected the amount of primary, secondary, tertiary follicle, total follicle and the amount of ovulation. The highest number of primary, secondary, tertiary follicle, total follicle and the number of ovulation obtained in P₇ were not significantly different from P₂ and K+, while the lowest was indicated by K-. Giving a combination of the three extracts affected the maturity of ovarian follicles, thereby increasing the fertility of female rats.