Physalis ixocarpa referred to as American Husk Tomato or Tomatillo was used as a vegetable and traditional medicine. It is potential for production mainly as functional foods. The objective of this study was to elucidate the development and character of flower Physalis ixocarpa genotype that expected scientific findings and valuable information for agricultural practices and conservation of plants. The study had used descriptive observational methods of flowering. The flowering stage consists of two stages. In the bud stage, the parameter observed was the first bud apperance time, the colour and size of buds and pedicels. In the blooming stage, the parameter observed was the colour and size of sepal, petal, pistil, anther and filament. The result found that each genotype had the same flower development stage. The flower character differences of each genotype included the first flower emerged time, the colour of the calyx, lateral anther, filament, and pedicels.