First detection of bovine tuberculosis by Ziehl–Neelsen staining and polymerase chain reaction at dairy farms in the Lekok Sub-District, Pasuruan Regency, and Surabaya region, Indonesia

Itfetania Aemilly Desire, Muhammad Luqman, Yulianna Puspitasari, Wiwiek Tyasningsih, Dhandy Koesoemo Wardhana, Dewa Ketut Meles, Yeni Dhamayanti, Dian Ayu Permatasari, Adiana Mutamsari Witaningrum, Agnes Dwi Sis Perwitasari, Hartanto Mulyo Raharjo, Siti Rani Ayuti, Shendy Canadya Kurniawan, Intan Noor Aina Kamaruzaman, Otto Sahat Martua Silaen

Research output: Contribution to journalArticlepeer-review

Abstract

Background and Aim: Bovine tuberculosis (TB) is a zoonotic disease of great public health importance, particularly in Indonesia, where control measures are limited or are not implemented. This study aimed to detect the presence of Mycobacterium pathogens in milk samples from dairy cattle in Pasuruan regency and Surabaya City, East Java, using Ziehl–Neelsen acid-fast staining and polymerase chain reaction (PCR). Materials and Methods: Milk samples were aseptically collected from 50 cattle in the Lekok Subdistrict, Pasuruan Regency, and 44 from dairy farms in the Lakarsantri Subdistrict, Wonocolo Subdistrict, Mulyorejo Subdistrict, and Kenjeran Subdistrict, Surabaya, East Java. To detect Mycobacteria at the species level, each sample was assessed by Ziehl–Neelsen staining and PCR using the RD1 and RD4 genes. Results: The results of PCR assay from 50 samples in Lekok Subdistrict, Pasuruan Regency showed that 30 samples (60%) were positive for Mycobacterium tuberculosis and two samples (4%) were positive for Mycobacterium bovis, although Ziehl–Neelsen staining did not show the presence of Mycobacterium spp. In the Surabaya region, 31 samples (70.45%) were positive for M. tuberculosis and three samples (6.8%) were positive for M. bovis. Six samples (13.63%) from all PCR-positive samples could be detected microscopically with Ziehl–Neelsen. Conclusion: The presence of bovine TB in this study supports the importance of using a molecular tool alongside routine surveillance for a better understanding of the epidemiology of bovine TB in East Java.

Original languageEnglish
Pages (from-to)577-584
Number of pages8
JournalVeterinary World
Volume17
Issue number3
DOIs
Publication statusPublished - Mar 2024

Keywords

  • bovine tuberculosis
  • cattle
  • polymerase chain reaction
  • public health
  • raw milk

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