Fibrin glue as an antifibrotic agent on human tenon fibroblast (HTFS) Through extracellular matrix expression

Human tenon fibroblast (HTFs) is the key componentof bleb fibrosis,followingthe process of trabeculectomy, andup to today, no ideal agent is capable of its inhibition. Furthermore, fibrin glue (FG) is a biomaterial that shows potentials in wound healing modulation, thus this study was aimed at investigatingitsantifibroticeffect on HTFs from glaucomatous eyes,expressed in term of Collagen 1, Matrix Metalloproteinase - 3 (MMP-3) and Tissue Inhibitor of Metalloproteinase - 2 (TIMP-2).HTF's from glaucomatous eyes were divided into 3 groups,which consists of FBS 2% as the control, MMC (Mitomycin C), and Fibrin Glue (FG) as treatment. Therefore, the experiment was conducted in 7 days, and the expressions of Col-1, MMP- 3, and TIMP-2 were assessed with immunofluorescence staining. In addition, the intensity of fluorescence was measured using ImageJ software, and the difference among groups were analyzed using the Oneway ANOVA test, followed by post-hoc, with the consideration of p<0.05 as significant. It was established that FG decreased the expression of Col-1and TIMP-2, as well as a subsequent increase in MMP-3. However, MMC was observed to possess the best antifibrotic effect in this study.FG exerts antifibrotic effect through the modulation of extracellular matrix.