TY - GEN
T1 - Fermentation of Rice Straw Raw Material to Bioethanol Using Consortium Enzyme
AU - Fithri, Lailatul
AU - Febriyanto, Luddy
AU - Asmarani, One
AU - Sanjaya, Rahmat Eko
AU - Nurrohman, Ardiana Ilham
AU - Lamid, Mirni
AU - Murad, Abdul Munir Abd
AU - Puspaningsih, Ni Nyoman Tri
N1 - Publisher Copyright:
© 2023 American Institute of Physics Inc.. All rights reserved.
PY - 2023/1/4
Y1 - 2023/1/4
N2 - Rice straw is one of the abundant lignocellulosic agricultural by-product in Indonesia. Rice straw has several characteristics that make it a potential raw material for bioenergy production such as ethanol. It contain high cellulose and hemicelluloses that can be hydrolyzed into fermentable sugars. This study aimed to hydrolyze rice straw cellulose and hemicellulose using xylanase, cellulose, and xylose isomerase enzymes. 2% H2SO4 was used in pretreatment before the rice straw hydrolyzed with consortium enzymes. Concentrations of rice straw substrate was made to 5%, 10%, 15%, and 20% (w/v) and also made 1:1, 1:2, and 1:3 volume ratio between substrate and enzymes. The results showed that the highest rate of bioethanol obtained from 10% (w/v) rice straw substrate and enzyme activity in consortium was 0.279 U/mL (xylanase), 0.329 U/mL (cellulase), and 0.024 U/mL (xylose isomerase). The hydrolyzate was fermented by Saccharomyces cerevisiae BJ1824 and produce 8.81x10-2 %(v/v) ethanol measured by Gas Chromatography (GC).
AB - Rice straw is one of the abundant lignocellulosic agricultural by-product in Indonesia. Rice straw has several characteristics that make it a potential raw material for bioenergy production such as ethanol. It contain high cellulose and hemicelluloses that can be hydrolyzed into fermentable sugars. This study aimed to hydrolyze rice straw cellulose and hemicellulose using xylanase, cellulose, and xylose isomerase enzymes. 2% H2SO4 was used in pretreatment before the rice straw hydrolyzed with consortium enzymes. Concentrations of rice straw substrate was made to 5%, 10%, 15%, and 20% (w/v) and also made 1:1, 1:2, and 1:3 volume ratio between substrate and enzymes. The results showed that the highest rate of bioethanol obtained from 10% (w/v) rice straw substrate and enzyme activity in consortium was 0.279 U/mL (xylanase), 0.329 U/mL (cellulase), and 0.024 U/mL (xylose isomerase). The hydrolyzate was fermented by Saccharomyces cerevisiae BJ1824 and produce 8.81x10-2 %(v/v) ethanol measured by Gas Chromatography (GC).
UR - http://www.scopus.com/inward/record.url?scp=85146498676&partnerID=8YFLogxK
U2 - 10.1063/5.0111387
DO - 10.1063/5.0111387
M3 - Conference contribution
AN - SCOPUS:85146498676
T3 - AIP Conference Proceedings
BT - 1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021
A2 - Kristiana, Arika Indah
A2 - Alfarisi, Ridho
PB - American Institute of Physics Inc.
T2 - 1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021
Y2 - 18 September 2021 through 19 September 2021
ER -