Fabrication of chitosan-chondroitin sulfate/hydroxyapatite composite scaffold by freeze drying method

Aminatun, Yunita Indriani, Dyah Hikmawati

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6 Citations (Scopus)


The use of tissue engineering technique increases along with the rise of bone tissue defect and bone tissue degeneration cases due to sickness, injury, trauma and accident. This study aimed to know the influence of variation from chondroitin sulfate (CS) and hydroxyapatite (HA) to scaffold characteristic and to determine the best composition for chitosan-chondroitin sulfate/hydroxyapatite (Ch-CS/HA) as bone scaffold candidate. The composition variation of Ch:CS:HA used in this study are A (35:0:65), B (35:5:60), C (35:10:55), D (35:15:50) and E (35:20:45) wt%. The synthesis of scaffold was done via freeze-drying method at-80 °C for 5 h and sublimation drying process for 48 h. The result of functional group test showed that the typical functional groups of HA, Ch and CS were simultaneously found at wave numbers of 962.48-1041.46 cm"1(P-OH), 1651.07-1658.78 cm"1(N-H), and 1417.68 cm"1(COO-). In SEM test, the pore diameter range obtained is 6.70-191.00 m. The result of porosity test from composite scaffold is 47.3408-82.869%. In vitro biodegradation test results showed that the highest percentage of weight loss in each sample, which was observed in week four, is 23.8619%. Cell Viability showed that more than 92.1514%. The compressive strength obtained is arpund 2.6914-7.6233 MPa which has met the standard as cancellous bone substitute. These characterizations suggest that sample B (35:5:60 wt%) is the best Ch-CS/HA composite as candidate for bone scaffold.

Original languageEnglish
Pages (from-to)1355-1362
Number of pages8
JournalJournal of International Dental and Medical Research
Issue number4
Publication statusPublished - 2019


  • Chitosan
  • Chondroitin sulfate
  • Hydroxyapatite
  • Scaffold


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