TY - JOUR
T1 - Fabrication and characterization of hydroxyapatite-polycaprolactone-collagen bone scaffold by electrospun nanofiber
AU - Yuwono, Luthfia Anindya
AU - Siswanto,
AU - Sari, Mona
AU - Yusuf, Yusril
AU - Suciati, Tri
AU - Sari, Yessie Widya
AU - Che Abdullah, Che Azurahanim
AU - Aminatun,
N1 - Publisher Copyright:
© 2022 Taylor & Francis Group, LLC.
PY - 2022
Y1 - 2022
N2 - According to the Indonesian Ministry of Health Research and Development Agency's 2018 Basic Health Research (Riskesdas), bone defects, because of fracture cases in Indonesia, reached a prevalence of 5.5%. Bone tissue engineering is one of the most promising new approaches for accelerating the growth and healing of bone defects in patients. In this study, a bone scaffold electrospun nanofiber composed of hydroxyapatite (HA)-polycaprolactone (PCL)-collagen was fabricated to mimic the extracellular matrix (ECM) found in native bone tissue and to promote bone remodeling and healing. Through characterization of functional groups with Fourier transform infrared (FTIR), morphology scanning electron microscopy (SEM), tensile strength test, degradation test, and cytotoxicity MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, this study determines the optimal composition of electrospun nanofiber HA/PCL/collagen to obtain the best candidate of a bone scaffold with excellent characteristics. Electrospinning was used to fabricate bone scaffolds. The samples used in this study consisted of control samples, namely PCL-HA and PCL-Collagen, and three samples with a mass ratio composition of HA-PCL-collagen 50:40:10, 50:30:20, and 50:25:25 (w/v%). The FTIR analysis of the sample revealed that no chemical bond existed between the materials. The HA/PCL/collagen 50:25:25 (F3) sample exhibited the best characteristics as a bone scaffold candidate, with a fiber diameter of 365 ± 202 nm; a porous fraction area of 58.98%; an ultimate tensile strength, and an elasticity modulus of 0.60 ± 0.185 and 5.98 ± 0.82 MPa, respectively; a degradation rate of 1.93 × 10−6 g/h; and cell viability of 81.03%.
AB - According to the Indonesian Ministry of Health Research and Development Agency's 2018 Basic Health Research (Riskesdas), bone defects, because of fracture cases in Indonesia, reached a prevalence of 5.5%. Bone tissue engineering is one of the most promising new approaches for accelerating the growth and healing of bone defects in patients. In this study, a bone scaffold electrospun nanofiber composed of hydroxyapatite (HA)-polycaprolactone (PCL)-collagen was fabricated to mimic the extracellular matrix (ECM) found in native bone tissue and to promote bone remodeling and healing. Through characterization of functional groups with Fourier transform infrared (FTIR), morphology scanning electron microscopy (SEM), tensile strength test, degradation test, and cytotoxicity MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, this study determines the optimal composition of electrospun nanofiber HA/PCL/collagen to obtain the best candidate of a bone scaffold with excellent characteristics. Electrospinning was used to fabricate bone scaffolds. The samples used in this study consisted of control samples, namely PCL-HA and PCL-Collagen, and three samples with a mass ratio composition of HA-PCL-collagen 50:40:10, 50:30:20, and 50:25:25 (w/v%). The FTIR analysis of the sample revealed that no chemical bond existed between the materials. The HA/PCL/collagen 50:25:25 (F3) sample exhibited the best characteristics as a bone scaffold candidate, with a fiber diameter of 365 ± 202 nm; a porous fraction area of 58.98%; an ultimate tensile strength, and an elasticity modulus of 0.60 ± 0.185 and 5.98 ± 0.82 MPa, respectively; a degradation rate of 1.93 × 10−6 g/h; and cell viability of 81.03%.
KW - Bone scaffold
KW - collagen
KW - electrospinning
KW - hydroxyapatite
KW - nanofiber
KW - polycaprolactone
UR - http://www.scopus.com/inward/record.url?scp=85134470026&partnerID=8YFLogxK
U2 - 10.1080/00914037.2022.2097675
DO - 10.1080/00914037.2022.2097675
M3 - Article
AN - SCOPUS:85134470026
SN - 0091-4037
JO - International Journal of Polymeric Materials and Polymeric Biomaterials
JF - International Journal of Polymeric Materials and Polymeric Biomaterials
ER -