Extraction of Bio-pigments from the Green Microalgae Chlorella pyrenoidosa under Different Solvent Ratios

Obie Farobie, Latifah Aisya Anis, Puji Rahmawati Nurcahyani, Edy Hartulistiyoso, Delicia Yunita Rahman, Widya Fatriasari, Ayu Lana Nafisyah, Apip Amrullah, Muhammad Aziz

Research output: Contribution to journalConference articlepeer-review

Abstract

Nowadays, the use of microalgae biomass is evolving rapidly for their various applications such as food, pharmaceutical, and cosmetics. One of the essential compounds in microalgae that have good perspectives in the market is pigment since it can be used as a natural colorant in food or cosmetics. However, the extraction of bio-pigments from the green microalgae Chlorella pyrenoidosa under different solvent ratios is still rarely reported. Therefore, this study aims to investigate the bio-pigment potential from C. pyrenoidosa extracted under different ethanol ratios. The algae-to-ethanol ratios of 1:5, 1:7.5, and 1:10 were employed in this study. Overall, C. pyrenoidosa contains total chlorophyll content much higher than carotenoids due to the fact that C. pyrenoidosa is the autotrophic microorganism that performs photosynthesis. The total chlorophyll content decreased with an increase in ethanol ratio, i.e., 134.47, 133.74, and 129.46 μg/mL at the ratio of 1:5, 1:7.5, and 1:10, respectively. In contrast, the carotenoid content slightly increased with an increase in ethanol ratio, i.e., 86.64, 87.08, 88.45 μg/mL at the ratio of 1:5, 1:7.5, and 1:10, respectively. The main chlorophyll compounds in the ethanol extract with the algae-to-ethanol molar ratio of 1:5 were identified as chlorophyll c (47.09 μg/mL), followed by chlorophyll b (38.50 μg/mL), chlorophyll a (28.67 μg/mL), and chlorophyll d (20.23 μg/mL).

Original languageEnglish
Article number012009
JournalIOP Conference Series: Earth and Environmental Science
Volume1187
Issue number1
DOIs
Publication statusPublished - 2023
Event7th International Conference on Biomass and Bioenerg, ICBB 2022 - Virtual, Online
Duration: 1 Aug 20222 Aug 2022

Keywords

  • Chlorellapyrenoidosa
  • algae
  • biomass
  • biorefinery
  • pigment

Fingerprint

Dive into the research topics of 'Extraction of Bio-pigments from the Green Microalgae Chlorella pyrenoidosa under Different Solvent Ratios'. Together they form a unique fingerprint.

Cite this