TY - JOUR
T1 - Expressions of Matrix Metalloproteinase-3 and Tissue Inhibitor Metalloproteinase-1 in Corneal Tissue Post Alkali Burn Treated with Topical Medroxyprogesterone Acetate and Doxycycline
AU - Bening, Daya Banyu
AU - Prastyani, Reni
AU - Nurwasis,
AU - Komaratih, Evelyn
AU - Zuhria, Ismi
AU - Notobroto, Hari Basuki
AU - Fauziah, Dyah
AU - Ardianto, Chrismawan
N1 - Funding Information:
FUNDING This article did not receive any specific grant of any funding.
Publisher Copyright:
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PY - 2023/7
Y1 - 2023/7
N2 - Purpose: This study aims to investigate the effects of topical Medroxyprogesterone acetate (MPA) and Doxycycline in inhibiting the expression of MMP-3 and TIMP-1 in ocular alkali burn models in animals. Methods: A total of 18 New Zealand Rabbits were divided into 3 groups based on their post-alkali-burn treatment: PBS (G1/ control group), topical Doxycycline 1mg/ml (G2), and topical MPA 1% (G3). Alkali burn models were made by exposing 1N NaOH solution to the central cornea for 30 seconds. MMP-3 and TIMP-1 expression were evaluated using immunohistochemistry after 14 days of treatment. Results: Statistically significant differences in the mean MMP-3 expression were found between the three groups (p=0.010). There was a significant difference in MMP-3 expression between the control group with MPA (p=0.017) and Doxycycline (p=0.028) but was not found between the MPA and Doxycycline groups (p=1,000). The mean differences in TIMP-1 expression between the three treatment groups were statistically significant (p=0.005), with a significant difference between the control group with Doxycycline (p=0.022) and MPA (p=0.007). There was no significant difference in TIMP-1 expression between the Doxycycline and MPA groups (P=1,000).
AB - Purpose: This study aims to investigate the effects of topical Medroxyprogesterone acetate (MPA) and Doxycycline in inhibiting the expression of MMP-3 and TIMP-1 in ocular alkali burn models in animals. Methods: A total of 18 New Zealand Rabbits were divided into 3 groups based on their post-alkali-burn treatment: PBS (G1/ control group), topical Doxycycline 1mg/ml (G2), and topical MPA 1% (G3). Alkali burn models were made by exposing 1N NaOH solution to the central cornea for 30 seconds. MMP-3 and TIMP-1 expression were evaluated using immunohistochemistry after 14 days of treatment. Results: Statistically significant differences in the mean MMP-3 expression were found between the three groups (p=0.010). There was a significant difference in MMP-3 expression between the control group with MPA (p=0.017) and Doxycycline (p=0.028) but was not found between the MPA and Doxycycline groups (p=1,000). The mean differences in TIMP-1 expression between the three treatment groups were statistically significant (p=0.005), with a significant difference between the control group with Doxycycline (p=0.022) and MPA (p=0.007). There was no significant difference in TIMP-1 expression between the Doxycycline and MPA groups (P=1,000).
KW - : Corneal alkali burn
KW - Doxycycline
KW - Medroxyprogesterone acetate
UR - http://www.scopus.com/inward/record.url?scp=85170529615&partnerID=8YFLogxK
U2 - 10.5530/pj.2023.15.118
DO - 10.5530/pj.2023.15.118
M3 - Article
AN - SCOPUS:85170529615
SN - 0975-3575
VL - 15
SP - 553
EP - 557
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 4
ER -