Expression of Nitrotyrosine and F2 Isoprostane after Administration of Lipopolysaccharide on Decreasing the Number of Dental Pulp Nerve Cells

Galih Sampoerno, Widya Saraswati, Devi Eka Juniarti, Noor Azlin Yahya, Gabriela Kevina Alifen, Daniyal Lazuardi Ramadhan, Muhammad Alviandi Hefni

Research output: Contribution to journalArticlepeer-review

Abstract

Dental caries can lead to inflammation or necrosis of the pulp, necessitating root canal treatment. However, this treatment carries the risk of pain, with its incidence influenced by the condition and quality of the pulp tissue. Immunologic and microbiologic factors play significant roles in determining the quality of the pulp tissue, thereby impacting the diagnosis of pulp tissue health. To elucidate the reduction in cell count resulting from the intrinsic apoptosis pathway of dental pulp nerve cells subsequent to lipopolysaccharide (LPS) administration, based on the expression levels of nitrotyrosine and F2-isoprostane. Thirty-two Sprague Dawley rats were divided into two groups. In the control group, only access opening was performed on the mandibular incisors of the rats. Conversely, in the treatment group, access opening was performed, and lipopolysaccharide (LPS) from Porphyromonas gingivalis was induced, left for 48 hours to induce an inflammatory condition, and subsequently terminated. Immunohistochemical staining was then employed to assess the expression of nitrotyrosine and F2-isoprostane. Additionally, the number of dental pulp nerve cells was observed using hematoxylin and eosin (HE) staining. Significant differences were observed in the expression levels of nitrotyrosine and F2- isoprostane, as well as in the number of nerve cells, between the control and treatment groups. Administration of lipopolysaccharide (LPS) leads to increased expression of nitrotyrosine and F2- isoprostane, while concurrently decreasing the number of dental pulp nerve cells affected by inflammation.

Original languageEnglish
Pages (from-to)632-637
Number of pages6
JournalJournal of International Dental and Medical Research
Volume17
Issue number2
Publication statusPublished - 2024

Keywords

  • apoptosis
  • F2 Isoprostane
  • immunology
  • LPS
  • nerve cells
  • Nitrotyrosine

Fingerprint

Dive into the research topics of 'Expression of Nitrotyrosine and F2 Isoprostane after Administration of Lipopolysaccharide on Decreasing the Number of Dental Pulp Nerve Cells'. Together they form a unique fingerprint.

Cite this