TY - JOUR
T1 - EXPRESSION OF ENDOGENOUS ANTIOXIDANT IN DENTINE PULP COMPLEX AFTER HEMA RESIN APPLICATION
AU - Saraswati, Widya
AU - Cahyani, Febriastuti
AU - Yonas, Yovita
AU - Gumilang, Saindra Arsa
AU - Dyah Kusumastuti, Yansha Mutia
AU - Dhaniar, Nina
AU - Praja, Hermawan Adi
N1 - Publisher Copyright:
© xxxx **
PY - 2020/10
Y1 - 2020/10
N2 - Free radicals can react with polyunsaturated acid in cellular membrane in DNA and caused cellular damage. In order to prevent tissue damage, reactive oxide metabolite should be eliminated through and enzymatic antioxidant such as superoxide dismutase (SOD), catalase, thioredoxin and glutathione peroxidase. Sprague Dawley rats were divided into 4 groups. 1 control group and 3 experimental groups. The tooth cavity in the control group was filled with glass ionomer cement without HEMA liquid resin. Tooth cavities in experimental groups were treated with HEMA liquid resin and then filled with glass ionomer cement. The teeth were extracted after 24, 48, and 72 hours. Immunohistochemistry staining was applied to investigate the expression of SOD and catalase. The observation was done under a light microscope. Test data were analyzed using the ANOVA test and Tukey HSD. Significant differences between SOD and Catalase were observed in the control group and experimental groups. There was no significant difference between the experimental groups (24, 48 and 72 hours). There was increased production of Catalase and SOD in dentine pulp complex after being applied with resin HEMA.
AB - Free radicals can react with polyunsaturated acid in cellular membrane in DNA and caused cellular damage. In order to prevent tissue damage, reactive oxide metabolite should be eliminated through and enzymatic antioxidant such as superoxide dismutase (SOD), catalase, thioredoxin and glutathione peroxidase. Sprague Dawley rats were divided into 4 groups. 1 control group and 3 experimental groups. The tooth cavity in the control group was filled with glass ionomer cement without HEMA liquid resin. Tooth cavities in experimental groups were treated with HEMA liquid resin and then filled with glass ionomer cement. The teeth were extracted after 24, 48, and 72 hours. Immunohistochemistry staining was applied to investigate the expression of SOD and catalase. The observation was done under a light microscope. Test data were analyzed using the ANOVA test and Tukey HSD. Significant differences between SOD and Catalase were observed in the control group and experimental groups. There was no significant difference between the experimental groups (24, 48 and 72 hours). There was increased production of Catalase and SOD in dentine pulp complex after being applied with resin HEMA.
KW - Superoxide dismutase
KW - catalase
KW - immunohistochemistry
KW - medicine
KW - odontoblasts
UR - http://www.scopus.com/inward/record.url?scp=85096460757&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85096460757
SN - 0972-5075
VL - 20
SP - 4809
EP - 4813
JO - Biochemical and Cellular Archives
JF - Biochemical and Cellular Archives
IS - 2
ER -